中文摘要：

不少研究表明，17α-乙炔基雌二醇（EE2）是雌情活力最强的类固醇雌激素。通过响应曲面法（RSM）优化EE2的高效液相色谱／串联质谱联用（HPLC／MS／MS）检测中的多离子检测反应（MRM）条件，获得EE2的最优碎片电压为182．0V，最优碰撞能为54．0V。配合固相萃取（SPE）预处理技术，采用HPLC／MS／MS内标检测法检测了重庆主城区嘉陵江表面水与污水水样中的EE2浓度，方法检测限为0．1ng／L；线性范围为0．51～99．37ng／mL；精密度试验中RSD为0．276％；重复性试验中RSD为8．788％；加标回收试验结果良好；单个混合样品分析周期为28．0min.

英文摘要：

The research shows that 17α-ethinyloestradiol （EE2） has the highest oestrogenic potential. The multiple reaction monitoring （MRM） conditions in determination method of HPLC-MS-MS for EE2 were optimized by the response surface method （RSM）. The optimal fragment voltage of EE2 is 182.0 V, and the collision energy is 54.0 V. After a sample pretreatment using two-step solid phase ex- traction （SPE） , this method was applied for the determination of EE2 in Jialing River surface water and sewage samples from Chongqing City. The detection limit is 0.1 ng/L, and the linear range is 0.51 to 99.37 ng/mL. The RSD in precision test is 0. 276%, and the RSD in repetitive experiments is 8. 788%. The average recovery is satisfactory, and the analysis cycle time of each sample for HPLC-MS-MS is 28.0 min.