中文摘要：

目的：单通道平面脂质双分子层电生理平台是目前研究离子通道特性最直接、最有效的技术手段。为扩展平台研究温度对离子通道电生理特性、通道稳定性等的研究的能力，本文借助循环水浴对平台的温控模块进行设计与实现。方法：本文以Warner公司的脂质双分子层电生理实验平台为操作平台搭建循环水浴温控模块。通过分析温控模块中循环水对平台电流测量的干扰并比较不同循环水排布方式对电流测量的干扰强度，我们设计了折返对偶排布的循环水排布回路，并借助α-溶血素七聚体大孔径离子通道检验该设计的实用效果。该排布方式能够降低循环水回路与电流测量回路间的电磁耦合，进而降低由循环水引入的电磁干扰，保证皮安级小电流的测量。结果：电流噪音可受循环水流回路的排布影响，其中在采用折返对偶排布的情况下，电流噪音与无温控系统空白对照的电流噪音无显著区别。结论：折返对偶排布的循环水流设计能够有效降低循环水温控模块对电流测量的电磁干扰。

英文摘要：

Objective： Single Channel Planar Lipid Bilayer Electrophysiology Platform is the most efficient and commonly used technique to study the characteristics of ion channels. In order to extend the platform to study the effects of temperature on the electrophysiology properties and stabilities of ion channels, we designed and implemented temperature control module for the platform. Methods： We build the circulation water bath temperature control module based on the Planar Lipid Bilayer Platform of Warner Instruments. With the analysis of the interference of water circulation path on current measurement and the experi- mental results of current noise of different circulation paths, we designed a turn-back dual circulation path. Further, we tested the usefulness of such design with a-hemolysin heptamer ion channel. Such design could reduce the electromagnetic coupling between water cycle path and current measurement circuit, so as to reduce the current noise brought by temperature control module. Results： The difference of the standard deviation of current noise with and without turn-back dual water path is quite small. Such design does not interference the measurement of current. Conclusions： Turn-back dual circulation path could reduce the negative effects of water control module on the measurement of current effectively.