中文摘要：

目的：探讨超高场强磁共振氢质子波谱（ hydrogen MR spectroscopy ，1 H-MRS）在全面评价ob/ob鼠及野生对照组小鼠白色脂肪组织和棕色脂肪脂质成分中的作用。方法：运用整体和单体素1 H-MRS方法对ob/ob及野生对照鼠进行活体波谱分析，整体1 H-MRS波谱定量全身脂质；单体素波谱计算白色和棕色脂肪的脂质含量，饱和及不饱和脂肪酸、双不饱和脂肪酸的相对含量及多聚不饱和程度。结果：整体1 H-MRS结果显示ob/ob鼠全身脂质含量显著高于野生型对照组，单体素1 H-MRS显示ob/ob鼠棕色脂肪脂质含量显著高于野生对照组，而两组鼠白色脂肪脂质含量的差异无统计学意义，ob/ob鼠白色脂肪的双不饱和脂肪酸及多聚不饱和程度均低于野生对照组。结论：1 H-MRS不仅可以定量白色和棕色脂肪的脂质含量，还可以分析其脂质成分。

英文摘要：

Objective： To evaluate hydrogen MR spectroscopy （ 1 H-MRS ） in estimating lipid content of whole body and measuring fat content in white and brown adipose tissue （ WAT and BAT ） of obese and normal mice in vivo.Methods：A single pulse 1 H-MRS and a localized proton 1 H-MRS were performed to measure the triglyceride from the whole body and the lipid contents in WAT and BAT of six ob/ob and six wild type（WT） mice using 7T micro-MR scanner . Fractions of unsaturated fatty acids （ FU ） , diunsaturated fatty acids （ FDU ） and polyunsaturation degree （ PUD） in WAT and BAT were calculated by using MRS .Independent-sample t test was performed in comparison .Results：In vivo total body spectroscopy showed the liquid lipid accumulation degree on the whole body in ob/ob mice was significantly higher than that of controls （ P〈0.001） .In vivo, BAT fat content in ob/ob mice measured by MRS was significantly higher than that of WT mice （ P 〈0 .001 ） , but no significant difference was found between ob/ob and WT mice in WAT fat content（P〉0.05）.PUD value and FDU of WAT in ob/ob mice were significantly lower than those of WT mice （ P〈0 .05 ） , but no significant difference was found between ob/ob and WT mice in FU of WAT and PUD value, FU, and FDU of BAT（P〉0.05）.Conclusion： 1H-MRS is efficient method in fat quantification at 7.0 T MR.In additions, MRS analyses PUD value and FU of WAT or BAT.They have the potential for further application quantization of lipid composition and lipometabolism in longitudinal studies .