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人脑胶质瘤裸鼠皮下移植瘤模型中血管生成拟态现象的观察
  • ISSN号:1001-2346
  • 期刊名称:《中华神经外科杂志》
  • 时间:0
  • 分类:R730.264[医药卫生—肿瘤;医药卫生—临床医学]
  • 作者机构:[1]中山大学肿瘤防治中心,华南肿瘤学国家重点实验室,广州510060
  • 相关基金:国家自然科学基金(30973478)
中文摘要:

目的观察胶质瘤裸鼠移植瘤生长过程中的血管生成拟态现象(VM)以及与血管内皮依赖性血管之间的关系。方法采用人脑胶质瘤细胞株U87单细胞悬液(2×10^6个/0.1ml)于4周龄裸鼠皮下注射建立移植瘤模型。应用血管内皮细胞标志物CD34和高碘酸雪夫(PAS)试剂双染的方法检测40例不同大小的皮下移植瘤中的VM和血管内皮依赖性血管。结果VM和血管内皮依赖性血管在不同大小的裸鼠皮下U87移植瘤中均存在。微血管密度(MVD)和肿瘤的直径呈正相关(r=0.393,P=0.012),血管生成拟态密度(VMD)与MVD负相关(r=-0.404,P=0.010),而与肿瘤的直径没有显著的相关性(P:0.575)。结论血管生成拟态与内皮依赖性血管是两种不同的肿瘤循环形式,在U87裸鼠皮下移植瘤的生长过程中持续存在并具有互补性,共同为肿瘤的生长提供营养支持。

英文摘要:

Objective This study was designed to observe the vasculogenic mimicry (VM)and the relationship between VM and endothelial - dependent vessels during the growth of subcutaneous xenograft glioma in Balb/c mice. Methods Forty 4 - week - old male Balb/c mice were inoculated with a single cell suspension of U87 (2 × 106 tumor cells were injected subcutaneously each mouse). The VM and endothelial - dependent vessels were detected by dual staining for endothelial marker CD34 and periodic acid - Schiff (PAS). The density of endothelium -dependent microvessels and VM was measured in tumor tissue sections by 5 randomly selected fields at 200 magnification and the average counts were then calculated. Results Endothelial -dependent vessels as well as VM in xenografts of U87 were persistent exist during the process of tumor growth. With the increase of tumor size, microvessel density (MVD) increased ( r =0. 393, P = 0. 012), while vasculogenic mimicry density (VMD) was no significant change (P =0. 575). The VMD were negative correlated with MVD ( r = -0. 404, P =0. 010). Conclusions There are two microcirculation patterns exist in xenografts of U87 in Balb/c mice during tumor growth, including VM and endothelial - dependent vessels. Both VM and endothelial - dependent vessels provide nutrition and oxygen for tumor development.

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期刊信息
  • 《中华神经外科杂志》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中华医学会
  • 主编:
  • 地址:北京市崇文区天坛西里6号
  • 邮编:100050
  • 邮箱:cjns65113169@sian.com
  • 电话:010-65113169
  • 国际标准刊号:ISSN:1001-2346
  • 国内统一刊号:ISSN:11-2050/R
  • 邮发代号:18-56
  • 获奖情况:
  • 1997年中国科协优秀科技期刊二等奖,1995年中华医学会成立80周年银奖
  • 国内外数据库收录:
  • 日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:39789