中文摘要：

目的：观察LED活化MPPa介导的光动力作用对人卵巢癌细胞生长的抑制作用。方法：采用倒置显微镜观察和MTT法检测LED活化MPPa光动力作用后人卵巢癌细胞株COC1/DDP细胞的生长状况。结果：LED活化MPPa介导的光动力作用显著抑制人卵巢癌细胞COC1/DDP细胞的生长,在一定浓度的MPPa条件下,细胞生长抑制作用和LED能量密度间呈剂量依赖关系。倒置显微镜下观察到LED活化MPPa介导的光动力作用组癌细胞数量显著减少,细胞固缩,而单纯LED照射组、单纯MPPa光敏剂处理组和假照射组三对照组间无明显差异;MTT法示LED活化MPPa介导的光动力作用组细胞抑制率显著高于单纯LED照射组、单纯MPPa组和假照射组（P〈0.01）,单纯LED照射组较假照射组有促进细胞增殖作用（P〈0.05）,而单纯MPPa光敏剂处理组和假照射组间无明显差异（P〉0.05）。结论：LED可以作为一种新型的光源活化MPPa等光敏剂介导的光动力作用从而有效抑制人卵巢癌细胞株COC1/DDP细胞生长。

英文摘要：

Objective：To explore photodynamic inhibition of Lighting Emitting Diode （LED） - activated pyropheophorbide- a methyl ester （MPPa） in human ovarian earcinoma cells. Methods： A special lighting emitting diode equipment was employed to investigate the photocytotoxic potency and efficacy of MPPa 24 h after PDT in a human ovarian carcinoma cell line COC1/DDP by inverted microscope and MTT reduction assay. Results：Inverted microscope and MTT reduction assay showed that the photodynamic inhibition of LED - activated MPPa in COC1/DDP was extraordinarily significant. The photocytotoxicity of MPPa indicated light-dose dependent characteristics in COC1/DDP cells, but COC1/DDP cells proliferation was promoted by LED irradiation alone. Conclusion： Lighting Emitting Diode （LED） - activated MPPa could effectively kill human ovarian carcinoma cells and LED will become an alternative light source for photodynamic therapy.