以膜荚黄芪无菌苗子叶节为外植体,建立了简单、高效和稳定的膜荚黄芪再生体系。结果表明:不定芽诱导的最适培养基是Ms+6-BA1.0nlg/L+NAA0.10mg/L,每个子叶节外植体平均出芽数为3个以上,再生频率达75.5%;生根最适培养基为1/2Ms+IBA0.5mg/L;移栽后成活率达75%以上。
The cotyledon node obtained from aseptic seedling of Astragalus mend)ranaceus were used as the explant and a simple,stable and high efficient regeneration system for A. membranaceus were established. The results showed that the optimum medium for adventitious shoot regeneration was MS+6-BA 1.0 mg/L+NAA 0. 10 mg/L,the average number of differential buds per cotyledon node was above 3,and the regenerated frequency was 75.5% . The suitable rooting medium was 1/2MS+IBA 0. 5 mg/L and the survive rate was above 75% after transplantation.