中文摘要：

目的观察脂氧素A4对椎间盘突出所致大鼠根性神经痛的影响。方法选取雄性SD大鼠48只，建立非压迫性椎间盘突出模型，按照随机数字表法将其分为假手术组（假手术＋10μl生理盐水）、对照组（模型＋10μl生理盐水）、脂氧素A。10ng组（模型＋10ng脂氧素A4）、脂氧素A4 100ng组（模型＋100ng脂氧素A4），每组12只。手术当天及术后连续3d鞘内注射脂氧素A4或生理盐水，观察大鼠的行为学变化，并测定50％缩足阈值（50％PWT）。术后7d取大鼠术侧腰段脊髓背角，用Westernblot法测定c—Jun氨基末端激酶（JNK）、细胞外信号调节激酶（ERK）的蛋白表达量，并用ELISA技术测定肿瘤坏死因子α（TNF—α）、白细胞介素1β（IL-1β）和转化生长因子-β1（TGF—β1）的含量。结果假手术组50％PWT在手术前后无显著变化（P〉0．05）。与组内术前比较，对照组和脂氧素A4 10ng组术后各时间点的50％PWT降低（P〈0．05）。与假手术组术后同时间点比较，对照组和脂氧素A4 10ng组术后各时间点的50％PWT较低（P〈0．05）。与对照组比较，脂氧素A4 10ng组术后3d[（8．90±2．76）g]、5d[（8．56±2．77）g]的50％PWT较高（P〈0．05）。脂氧素A4 100ng组术后2d、3d、4d、5d、6d、7d的50％PWT显著较高（P〈0．05）。与假手术组比较，对照组、脂氧素A4 10ng组和脂氧素A4 100ng组p-JNK、p-ERK水平明显升高（P〈0．05）。与对照组比较，脂氧素A4 10ng组和脂氧素A。100ng组p-JNK、p-ERK水平明显降低（P〈0．05），且脂氧素A4 100ng组下降更明显（P〈0．05），呈剂量依赖性。各组t-JNK和t-ERK蛋白含量之间比较，差异无统计学意义（P〉0．05）。与假手术组比较，对照组、脂氧素A4 10ng组和脂氧素A4 100ng组TNF-α、IL-1β的表达水平明显升高，TGF-β1的表达水平明显降低（P〈0．05）。与对照组比较，脂氧素A4 10ng组和脂氧素A4 100ng组TNF

英文摘要：

Objective To investigate the effect of lipoxin A4 （LXA4） on radicular pain caused by intervertebral disc herniation. Methods Non-compressive intervertebral disc herniation was induced into forty-eight adult male Sprague-Dawley rats, and they were divided into a sham group （sham operation ＋ 10 μl normal saline） , a control group （modeled ＋ 10 μl normal saline） , an LXA4 10 ng group （modeled ＋ 10 ng LXA4） and an LXA4 100 ng group （modeled ＋ 100 ng LXA4） , with 12 rats in each group. The normal saline （ 10 μl） or LXA4 （ 10 μl） was administered intratheeally right after the operation and on each of the three succeeding days. General behavior was observed and the 50% paw withdrawal threshold （50% PWT） was measured. On postoperative day 7 all the rats were killed and the ipsilateral lumbar （ L4-6 ） segments of their spinal dorsal horns were removed for de- termination of the expression of p-JNK, t-JNK, p-ERK and t-ERK proteins using western blotting. TNF-α, IL-1β and TGF-β1 expression were determined using ELISA. Results There was no significant difference in the 50% PWT of the sham group before and after surgery, but the 50% PWTs of the control group and the LXA4 10 ng group were significantly decreased after the operation compared with their values beforehand and significantly lower than the value of the sham group at all time points. Moreover, the 50% PWT of the LXA4 10 ng group on postoperative days 3 and 5 was significantly higher than the control group; as was the value of the LXA4 100 ng group on postoperative days 2, 3, 4, 5, 6 and 7. The p-JNK and p-ERK expression in the control group, the LXA4 10 ng group and the LXA4 100 ng group were all increased significantly more than in the sham group, but their expression in the LXA4 10 ng group and LXA4 100 ng group were decreased significantly more in a dose-dependent manner compared with the control group, with the LXA4 100 ng group showing the greatest decrease. There were no significant differences in t-JNK or t-E