中文摘要：

应用SDS—PAGE电泳检测方法，研究了本实验室构建的人TRIM5α嵌合体重组蛋白在大肠杆菌中的最佳表达备件。结果表明：在31℃、IPTG的诱导浓度为0．5mmol／L、诱导时长为8h、菌液的OD值为0．6以及在TB培养基上培养条件下人TRIM5α嵌合体蛋白表达量达到最大。

英文摘要：

The optimum conditions for the expression of human TRIM5 chimera recombinant protein which was constructed in our lab in E. coli DE3 were studied by using SDS - PAGE electrophoresis method. The results indicated that the maximal expression of human TRIM5α chimera.recombinant plasmid was induced by 0.5 mmol/L IPTG at 31℃ for 8 h when E. coli was raised on TB culture medium and the OD - value of bacterium solution was 0.6.