中文摘要：

目的：构建稳定表达细胞外信号调节激酶（ERK1／2）的骨髓间充质干细胞（MSCs）及探讨其对脑卒中的神经保护作用。方法：制备ERK1／2重组病毒和空病毒（对照），并转染大鼠MSCs，分别构建ERK1／2／MSCs和BV／MSCs（对照）。用RT-PCR和免疫印迹检测ERK1／2／MSCs的ERK1／2表达水平。制备大鼠脑卒中模型，移植ERK1／2／MSCs、BV／MSCs和生理盐水后1、2周，观察动物的神经行为症状和TTC染色观察脑梗死体积。结果：构建了ERK1／2的重组慢病毒质粒，并转染MSCs，RT—PCR和免疫印迹检测显示ERK1／2／MSCs细胞系过表达ERK1／2。大鼠脑卒中后移植ERK1／2／MSCs、BV／MSCs和生理盐水（对照组），ERK1／2／MSCs和BV／MSCs组的大鼠1、2周时，神经功能的恢复显著好于对照组，而ERK1／2／MSCs比BV／MSCs有更好的效果；ERK1／2／MSCs和BV／MSCs的大鼠脑梗死的体积比对照组显著降低，而ERK1／2／MSCs和BV／MSCs两组之间没有差异；移植后2周，ERK1／2／MSCs组的大鼠移植细胞存活数比BV／MSCs组的多。结论：本实验成功构建了稳定表达ERK1／2的MSCs，其对脑卒中的神经保护效果优于MSCs。

英文摘要：

Objective： To construct marrow stroma cells stably overexpressing extracellular singal-regulated kinase 1/2 （ERK1/2/MSCs） and explore whether transplantation of ERK1/2/MSCs provides a better therapeutic for rat stroke. Methods： After the resultant ERK1/2 recombinant viruses were amplificated in colibacillus and were packaged in 293FT, ERK1/2 and blank virus solution was got. After MSCs were transfected by ERK1/2 and blank virus solutions （ERK1/2/MSCs, BV/ MSCs）, RT-PCR and Western blotting were used to detect the ERK1/2 expression. The ischemic stroke model was established by occluding the middle cerebral artery. At 3 days after reperfusion, ERK1/2/MSCs, BV/MSCs and physiological saline （control） were infused into ipsilateral ventricle for 1 and 2 weeks. Neural behavior and infarct volume was detected. Results： The ERK1/2 recombinant virus was successfully constructed, and then MSCs were transfected by ERK1/2 recombinant virus and blank virus （ERK1/2/MSCs, BV/MSCs）. RT-PCR and Western blotting showed that ERK1/2/MSCs over-expressed ERK1/2 compared to BV/MSCs and native MSCs. At 1 and 2 weeks after transplantation, ERK1/2/MSCs and BV/ MSCs groups significantly improved neural behavioral deficits compared to the rats treated with physiological saline （control）, and ERK1/2/MSCs showed more effective effects than BV/MSCs. ERK1/2/MSCs and BV/MSCs significantly decreased infarct volume compared to the control rats at the given time, while ERK1/2/MSCs did not show significant difference as compared to BV/MSCs. The survival of grafted ceils in ERK1/2 group was significantly increased as compared to BV/MSCsgroup at 2 weeks after reperfusion. Conclusion： The present study successfully constitutes MSCs stably over-expressing ERK1/2, which provide better neuroprotection than BV/MSCs against stroke.