中文摘要：

在土壤上估计镉( Cd )和水银( Hg )的单个、联合的污染的效果结构的微生物引起的社区和功能的差异，一个孵化实验被进行，也就是，由采用二玷污海洋的沉积淤泥的沃土土壤和微黄色红的土壤，在哪个 Cd ， Hg 和在联合的 Hg 的五个层次被增加。在被孵化 56 天以后，丰满的酸(PLFA ) 介绍的 phospholipid 和样品的唯一的碳来源利用模式(BIOLOG ) 被测试。结果证明微生物引起的社区的作文在金属申请的不同层次显著地变化了。PLFA 的主要部件分析(PCA ) 显示微生物引起的社区的结构显著地也与增加金属的层次被改变，与增加为真菌和放射菌类的 PLFA biomarkers，并且增加比率对克否定的细菌克积极。唯一的碳来源利用模式分析揭示了 Cd 的那单个、联合的应用， Hg 显著地禁止了土壤微生物的功能的活动，功能的差异索引[丰富(S)，Shannon牛肉熏香肠索引(H)和平均( E H )]比在非弄脏的土壤的那些在弄脏的土壤是显著地更低的，它显著地也与增加金属的层次改变了。为唯一的碳来源利用模式的 PCA 也显示金属污染能导致可变土壤微生物引起的社区。结果表明 Cd 和 Hg 的联合有更高的毒性比 Cd 或 Hg 的单个申请玷污结构的微生物引起的社区和功能的差异。

英文摘要：

To assess the effects of single and combined pollution of cadmium （Cd） and mercury （Hg） on soil microbial community structural and functional diversities, an incubation experiment was conducted, by employing two soils, namely, the marine sediment silty loam soil and the yellowish-red soil, in which five levels of Cd, Hg and Cd and Hg in combination were added. After being incubated for 56 days, the phospholipid fatty acids （PLFAs） profile and sole carbon source utilization pattern （BIOLOG） of the samples were tested. The results showed that the composition of the microbial communities changed significantly at different levels of metals application. The principal component analyses （PCA） of PLFAs indicated that the structure of the microbial community was also significantly altered with increasing levels of metals, with increasing PLFAs biomarkers for fungi and actinomycetes, and increasing ratio of Gram-positive to Gram-negative bacteria. Sole carbon source utilization pattern analysis revealed that single and combined application of Cd and Hg inhibited significantly the functional activity of soil microorganisms, the functional diversity indices [Richness （S）, Shannon-Wiener indices （H） and Evenness （EH）] were significandy lower in polluted soils than those in non-polluted soils, which also significantly altered with increasing levels of metals. PCA for the sole carbon source utilization pattern also indicated that the metal contamination could result in a variable soil microbial community. The results revealed that the combination of Cd and Hg had higher toxicity to soil microbial community structural and functional diversities than the individual application of Cd or Hg.