中文摘要：

Thioredoxin (Trx ) 蛋白质特别涉及许多生物过程细胞的氧化还原作用动态平衡的规定。在这研究，二 Trx cDNAs 用 cDNA 结束聚合酶链反应(RACE-PCR ) 的快速的 amplifi 阳离子从贻贝 Mytilus galloprovincialis 被克隆。二 cDNAs 分别地被称为 MgTrx1 和 MgTrx2。MgTrx1 和 MgTrx2 的开的读物框架是 318 和 507 基础对(bp ) ，他们分别地与 11.45 和 18.93 kDa 的估计的分子的群众编码了 105 和 168 氨基酸的蛋白质。顺序分析表明两蛋白质拥有了保存活跃地点 dithiol 主题 Cys-Gly-Pro-Cys。另外， MgTrx2 也拥有了指向建议它位于线粒体的信号的通常认为的 mitochondrial。量的即时聚合酶链反应(qPCR ) 表明 MgTrx1 和 MgTrx2 组成地在检验的所有纸巾被表示。而 MgTrx2 抄本很充满性腺， hepatopancreas，鳃和血球， MgTrx1 抄本很充满血球和鳃。后面的 Vibrio anguillarum 挑战， MgTrx1 的表示是起来调整的并且到达了它的山峰，在价值 10 褶层起始的价值，在 24 h。随后，表示回来了回到原来的水平。相反， MgTrx2 的表达式水平是下面调整的跟随细菌的刺激，与在 12 h 帖子挑战明显的控制水平的一 fi fth。这些结果建议 MgTrx1 和 MgTrx2 可以在 M 的反应起重要作用。galloprovincialis 到细菌的挑战。

英文摘要：

Thioredoxin （Trx） proteins are involved in many biological processes especially the regulation of cellular redox homeostasis. In this study, two Trx cDNAs were cloned from the mussel Mytilus galloprovincialis using rapid amplification of cDNA ends-polymerase chain reaction （RACE-PCR）. The two cDNAs were named MgTrxl and MgTrx2, respectively. The open reading frames of MgTrxl and MgTrx2 were 318 and 507 base pairs （bp） and they encoded proteins of 105 and 168 amino acids with estimated molecular masses of 11.45 and 18.93 kDa, respectively. Sequence analysis revealed that both proteins possessed the conserved active site dithiol motif Cys-Gly-Pro-Cys. In addition, MgTrx2 also possessed a putative mitochondrial targeting signal suggesting that it is located in the mitochondria. Quantitative real-time polymerase chain reaction （qPCR） revealed that both MgTrxl and MgTrx2 were constitutively expressed in all tissues examined. The MgTrxl transcript was most abundant in hemocytes and gills, whereas the MgTrx2 transcript was most abundant in gonad, hepatopancreas, gill and hemocytes. Following Vibrio anguillarum challenge, the expression of MgTrxl was up-regulated and reached its peak, at a value 10-fold the initial value, at 24 h. Subsequently, expression returned back to the original level. In contrast, the expression level of MgTrx2 was down-regulated following bacterial stimulation, with one fifth of the control level evident at 12 h post challenge. These results suggest that MgTrxl and MgTrx2 may play important roles in the response ofM. galloprovincialis to bacterial challenge.