中文摘要：

目的探讨碘油羟基磷灰石纳米微粒（nHAP）经肝动脉治疗对兔VX2肝肿瘤细胞凋亡的影响及机制。方法100只新西兰白兔肝内肿瘤种植后2周,随机分为5组,每组20只,经肝动脉灌注给药,实验设生理盐水组（A组）、nHAP组（B组）、单纯碘油组（C组）、阿霉素碘油组（D组）及碘油nHAP组（E组）。治疗2周后,流式细胞术检测肝肿瘤组织中细胞凋亡,并利用Western blot检测凋亡抑制相关蛋白XIAP及Survivin的表达。结果流式细胞术检测肝肿瘤组织细胞凋亡结果显示,A组和B组比较无明显差异,C组和D组残余肿瘤区域细胞凋亡率较A组升高（P〈0.05）,而E组细胞凋亡率较C、D两组明显升高（P〈0.05）。Westernblot结果提示与A组相比,C组和D组残余肿瘤区的XIAP和Survivin表达降低（P〈0.05）,E组XIAP和Survivin蛋白表达较C、D两组降低（P〈0.05）。结论碘油羟基磷灰石纳米微粒可进一步促进肝脏肿瘤细胞凋亡,这种作用可能与碘油nHAP降低凋亡抑制蛋白XIAP和Survivin表达有关。

英文摘要：

Objective To study the effect and mechanism of lipiodol-hydroxyapatite nanoparticle（nHAP） on apoptosis in rabbit VX2 hepatic tumor.Methods One hundred New Zealand white rabbits with VX2 carcinoma implanted in the left lobes of hepar for two weeks were randomly divided into 5 groups,each 20 cases.Every group was infused via the hepatic artery the physiological saline （group A）,nHAP（group B）,Lipidol（group C）,ADM adding Lipiodol（group D） and Lipi-nHAP（group E）.The apoptosis rate was examined by Flow cytometry. Expression of inhibiting apoptosis protein XIAP and Survivin gene were detected by Western blotting investigation.Results Apoptosis rates of group C and D were higher than group A（P0.05）.The apoptosis rate in group E was notable higher than group C and D（P0.05）.The result of western blot demonstrated that the expression of XIAP and Survivin protein in group C and D were lower than those in group A（P0.05）.The expression of XIAP and Survivin protein were lower in group E than those in group C and D（P0.05）.Conclusion nHAP can enhance the apoptosis effect of VX2 tumor.And this effect may involve the expression of XIAP and Survivin proteins.