中文摘要：

目的评估化合物1-[4’-（（3R，5R）-金刚烷基）苯基）]正丁胺盐酸盐（T44）的体外抗肿瘤活性，并初步探索可能的分子机制，为这种化合物的综合应用提供实验依据。方法四甲基偶氮唑蓝比色法检测T44对细胞的体外生长抑制作用；细胞形态观察法研究T44对细胞形态的影响；流式细胞仪法检测T44作用下肿瘤细胞周期和凋亡率的变化；蛋白印迹法检测T44对不同周期蛋白表达的影响；细胞划痕实验检测T44对肿瘤细胞迁移能力的影响。结果T44对检测的11株肿瘤细胞的增殖均有抑制效果，对正常细胞人胚肺成纤维细胞也具有一定的增殖抑制作用，半数抑制浓度值为（16．58±3．49）μmol／L，高于受测的大部分肿瘤细胞；T44能导致肿瘤细胞凋亡和周期阻滞；T44能影响周期蛋白的表达并能降低HCT116细胞的迁移能力。结论T44主要通过促进细胞凋亡来发挥抗肿瘤作用。

英文摘要：

Objective In order to provide experimental evidence for the comprehensive application of compound T44, we explored the possible mechanisms of its anti-tumor effects. Methods MTY assay was used to measure cell proliferation. Transition of cell cycle and apoptosis were tested by flow cytometer, respectively. Capacity of cell migration was detected by scratch assay. Western blotting was applied for determining the expression of different cyclins. Detection of cell morphology change was utilized to observe the influence on HCT116 cells. Results The proliferation of 11 kinds of tumor cell lines were decreased markedly as compared with those of control cells, and they showed distinct sensibility with one another. Half maximal （50%） inhibitory concemtration of human lung fibroblasts was （ 16.58 ± 3.49） μmoL/L, higher than most of the tumor cell lines. T44 treated HCT116 cells decreased cell migration and altered cyclins expression, as well. T44 could also lead to cell cycle block and apoptosis. Conclusion T44 participated into the control of tumor proliferation probably by modUlation of cell cycle and induction of apoptosis.