采用扩增片段长度多态性AFLP标记对国内外31个杧果主要品种进行分类与亲缘关系研究。结果表明:筛选出的14对引物组合在31份杧果种质中共扩增出了1761条带,其中多态性带的比例为97%。平均每对引物产生125.8条带和121.6条多态性带,总的多态性带率为97%;基于AFLP标记,以0.48的相似系数为阈值,所有供试杧果材料被分为7大组群,第一组群内以0.52为阈值,又可分为6组。与形态标记相比,基于AFLP标记的杧果分类体系更能反映杧果品种间的亲缘关系。
In this article, the classification and the relatio (Mangifera indica Linn. ) cuhivars were investigated via amp nships of 31 main domestic and overseas mango lifted fragments length polymorphism (AFLP) markers. The results showed that the 14-pair-prlmer combination screened totally amplified 1 761 bands from the sampled mango cuhivars, and 97% of all bands were polymorphic. For each pair of primers, there were 125.8 bands and 121.6 polymorphic bands and the polymorphic bands rate was 97%. Based on the AFLP markers, all the materials were divided into 7 groups with the threshold of O. 48 of similarity coefficient. At the same time, the first group was divided into 6 subgroups with the threshold of 0. 52 of similarity coefficient. Compared with morphologic markers, the mango classification system based on AFLP markers could reflect relationship among mango cuhivars more efficiently.