中文摘要：

为纯化茅岩莓总黄酮,先用HPD-100型大孔吸附树脂（macroporous adsorption resin,MAR）层析柱进行初步纯化,再用聚酰胺（polyamide,PA）层析柱进行第2次纯化,得到的HPD-100型MAR最适宜吸附工艺参数为上样液总黄酮质量浓度6 mg/mL、上样流速1 mL/min、上样液体积130 mL,在此条件下吸附率为97.14%;最适宜解吸工艺参数为洗脱液乙醇体积分数70%、洗脱流速1 mL/min、洗脱液体积40 mL,在此条件下解吸率为94.10%。经HPD-100型MAR纯化后的总黄酮纯度从55.00%提高到了72.25%。PA的最适宜吸附工艺参数为上样液总黄酮质量浓度6 mg/mL、上样流速2 mL/min,在此条件下吸附率为99.57%;最适宜解吸工艺参数为洗脱液乙醇体积分数70%、洗脱流速1 mL/min、洗脱液体积55 mL,在此条件下解吸率为76.50%。经PA纯化后总黄酮纯度从72.25%提高到了80.75%。该方法为茅岩莓黄酮的纯化提供了一种更高效的方法,具有良好的应用前景。

英文摘要：

Sequential column chromatographies on HPD-100 macroporous adsorption resin （MAR） and polyamide （PA）were used to purify total flavonoids from Ampelopsis grossedentata. The optimum adsorption parameters for HPD-100 MARwere determined as follows： sample concentration, 6 mg/mL; sample flow rate, 1 mL/min; and sample volume, 130 mL.Under these conditions, the percentage adsorption was 97.14%. The optimum desorption parameters were established asfollows： eluent concentration, aqueous ethanol （70：30, V/V）; eluent volume, 40 mL; and flow rate, 1 mL/min. Under theseconditions, the percentage desorption was 94.10%. After the treatment with HPD-100 MAR, the purity of total flavonoidswas increased from 55.00% to 72.25%. The optimum adsorption parameters for PA were determined as follows： sampleconcentration, 6 mg/mL; and flow rate, 2 mL/min. Under these conditions, the percentage adsorption was 99.57%. Theoptimum desorption parameters were determined as follows： 70% ethanol in water （V/V） as the eluent; eluent volume,55 mL; and flow rate, 1 mL/min. Under these conditions, the percentage desorption was 76.50%. After the treatment withPA, the purity of total flavonoids was further increased to 80.75%. The method presented in this study can provided apromising approach for the efficiency purification of total flavonoids from Ampelopsis grossedentata.