中文摘要：

通过从中国虾(Fenneropenaeus chinensis ) 的随机的染色体序列探索微卫星教材，一些微卫星教材与富有的多态的遗传信息被获得，并且三重的 PCR 用三份教材(RS1101， RS0683 和 H081 教材) 被建立。由调整 Mg~(2+) 的最后的集中， dNTP 和教材，并且用一个触摸镇 PCR 程序， PCR 系统的最佳扩大参数被获得，它能成功地在 PCR 反应放大三份教材。在使中毒的页胶化，三教材 RS1101， RS0683 和 H081 的放大 DNA 碎片能是容易识别了对方。为三重的 PCR 系统， PPE (渊源排除的可能性) 是 0。967 9，并且 DP (辨别力量) 是 0。999 327。用到出身和他们的父母的测试十个人的三重的 PCR，一个个人 threemicrosatellite loci 在所有从出身被排除，它可能为象虚假失误那样的一些未知理智被混合进出身。三重的 PCR 将具有在识别 F 的出身的大实际价值。chinensis。

英文摘要：

Through exploring the microsatellite primers from the random genome sequences of Chinese shrimp （Fenneropenaeus chinensis）, some microsatellite primers were obtained with rich polymorphic genetic information, and a triplex PCR was established using three primers （RS1101, RS0683 and H081 primers）. By adjusting the final concentration of Mg^2＋, dNTP and primers, and using a touch-town PCR program, the optimum amplification parameters of PCR system were obtained, which could successfully amplify the three primers in a PCR reaction. In the denatured PAGE gel, the amplified DNA fragments of three primers RS1 101,RS0683 and H081 could be easily identified each other. For the triplex PCR system, the PPE （probabilities of paternity exclusion） is 0.967 9,and the DP （discrimination power） is 0.999 327.Using the triplex PCR to test ten individuals of a parentage and their parents, an individual was excluded from the parentage in all of the three microsatellite loci, which might be mixed into the parentage for some unknown reason such as factitious misplay. The triplex PCR will be of great practical value in identifying the parentages of F. chinensis.