文章对相同条件下培养的莱茵衣藻野生型CC-137和八氢番茄红素脱氢酶(phytoene desaturase,PDS)基因突变株Nfr-4的生长进行分析;并用反相高效液相色谱分析总有色类胡萝卜素以及叶绿素含量变化,结果表明两者生长的差异明显;Nfr-4突变株的单细胞叶绿素和总有色类胡萝卜素含量高于野生型CC-137的.
The differences of the growth curves and total contents of coloured carotenoid and chlorophyll between Chlamydomonas reinhardtii wild-type CC-137 and mutant strain Nfr-4 were analyzed respectively. The changes in contents of carotenoid and chlorophyll were detected by revese phyase-HLPC. It was demon- strated that the total amount of chlorophyll and total coloured carotenoid were higher in mutant Nfr-4 than those in wild-type CC-137 under identical growth conditions, and the growth curves of the two strains were evidently different.