中文摘要：

为检测家蝇对拟除虫菊酯击倒抗性kdr等位基因上的L1014F点突变，采用了寡核苷酸探针法（PCR-Allele Specific Oligo nucleotide probe，PCR-ASO），用于检测该点突变。对L1014位的上下游DNA进行PCR扩增，并将纯化产物平行点样在带正电荷的尼龙膜上，用2条地高辛标记的特异性寡核苷酸探针，1条为敏感型，1条为突变型，与之杂交，根据显影的阳性信号判断待检样品的kdr等位基因型。该方法能区分敏感纯合子、抗性纯合子及杂合子，是检测家蝇kdr抗性的有用工具。

英文摘要：

PCR-ASO （PCR-Allele Specific Oligo nucleotide probe） method was used to detect L1014F mutation associated with kdr resistance of house fly, Musca domestica. Genomic DNA fragment including L1014 from individual of house fly was amplified by PCR and transferred to duplicated Hybond-N^＋ membranes, which was hybridized with a susceptible probe and a mutated probe marked with DIG respectively. The kdr genotype such as susceptible homozygote, resistant homozygote and heterozygote was then identified by the result of positive signal. PCR-ASO was a useful tool for detecting kdr resistance of house fly.