中文摘要：

目的观察低氧环境对子宫内膜异位症（EMs）的在位细胞葡萄糖、乳酸及三磷酸腺苷（ATP）的改变,探讨低氧在EMs发病机理中的作用。方法收集10例EMs患者在位内膜（A组）和10例正常内膜组织（B组）,分别置于常氧（21%O2）与低氧（1%O2）培养箱中48h,测定葡萄糖消耗、乳酸及ATP的生成。结果常氧下,两组细胞葡萄糖消耗、乳酸及ATP生成水平相似（P〉0.05）。低氧下,A组细胞葡萄糖消耗和乳酸生成均高于B组[（14.58±3.59）mmol·L^-1·10^-6cell vs.（8.58±5.54）mmol·L^-1·10^-6cell和（0.99±0.19）mmol·L^-1·10^-6cell vs.（0.58±0.29）mmol·L^-1·1^0-6cell]（P〈0.05）,而ATP生成水平相仿[（8.72±0.35）nmol×10^6cell vs.（8.70±0.53）nmol×106cell）（P〉0.05）]。结论低氧可能提高EMs在位内膜的葡萄糖消耗及乳酸生成,从而可能有利于EMs的疾病发展。

英文摘要：

Objective To observe the changes of glucose, lactate and adenosine triphosphate （ATP） in eutopic endometrial stromal cells from women with endometriosis（EMs） under low oxygen tension and investigate the role of hypoxia in the pathogenesis of EMs. Methods Stromal cells were isolated from endometrium in ten women with EMs（group A） and ten without（group B）. The tissues were digested and cultured to get the cells, which then were placed in 21% O2 and in 1% O2 respectively, and incubated for 48 hours. The glucose consumption, lactate generation and ATP. production were determined. Results There were no significant differences in the glucose consumption, lactate and ATP production of endometrial cells in 21%O2 between two groups （P〉0.05）. The glucose consumption and lactate production of the cells in 1% 02 were more in group A than those in group B[（14.58±3.59） mmol·L^-1·10^-6cell vs. （8. 58±5.54） mmol·L^-1·10^-6cell and （0.99±0.19） mmol·L^-1·10^-6 eell vs. （0.5±0.29） mmol·L^-1·10^-6 cell]（P〈0.05） ,hut ATP production of both groups was similar[（8.72±0. 35） nmol× 10^6 cell vs （8. 70±0. 53） nmol× 10^6 cell） （P〉0. 05）]. Conclusion Low oxygen may promote the glucose consumption and lactate production of eutopic cells,which may take a role in the pathogenesis of EMs.