中文摘要：

目的预测衣原体噬菌体phiCPG1衣壳蛋白Vp1的空间结构，并将Vp1表达为更小的蛋白片段，为后续实验奠定基础。方法通过蛋白空间结构分析网站I-TASSER和PredictProtein对phiCPG1衣壳蛋白Vp1的空间结构进行预测，应用TA克隆的方法将Vp1分为不同的部分进行克隆表达，最后通过Westernblot技术分别对目的蛋白进行鉴定。结果根据空间结构预测结果以及相关背景资料将衣原体噬菌体phiCPG1衣壳蛋白Vp1分为Vp11-189和Vp1190502两部分进行克隆表达，目的蛋白Vp11-189和Vp1190-502的基因序列长度分别为567bp和939bp，经检索确定两蛋白碱基序列与Genebank结果相同。而且，Westernblot结果显示两目的蛋白的相对分子质量分别为20kDa和35kDa。结论成功将衣原体噬菌体phiCPG1衣壳蛋白Vp1分为Vp11189和Vp1190502两部分进行表达，这为后期的深入研究提供了一定的实验基础。

英文摘要：

Objective To predict the spatial structure of the chlamydiaphage phiCPG1 capsid protein Vpl,and to produce smaller-sized protein in order to provide a basis for further studies. Methods The websites I-TASSER and PredictProtein were used to predict the spatial structure of the chlamydiaphage phiCPG1 capsid protein Vpl. Various fragments of chlamydiaphage phiCPG1 capsid protein Vpl were cloned with TA-cloning technique, and identified by Western blot. Results According to the predicted results and the relevant background information, Vp1 1-189 and Vp1 190-502 were cloned. The sequence sizes of Vp1 1-189 and Vp1190-502 genes were 567bp and 939bp, respectively,which base sequences were the same as that in the Genebank. The Western blot revealed that the relative molecular weight of Vp1 1-189 and Vp1 190-502 were 20kDa and 35kDa respectively. Conclusion Two fragments chlamydiaphage phiCPG1 capsid protein Vp1, Vp1 1-189 and Vp1 190-502, have been cloned, providing a valuable reference for future studies.