中文摘要：

规模化猪场猪繁殖与呼吸综合征病毒（PRRSV）呈持续性感染，给养猪业造成的损失最为严重。部分规模化猪场未实施切实的猪繁殖与呼吸综合征疫苗免疫，呈慢性和潜伏感染，作者现场对妊娠母猪接种高致病性猪繁殖与呼吸综合征（HP-PRRS）减毒活疫苗（TJM-F92株），旨在从体液免疫、细胞免疫、非特异性免疫功能角度探讨妊娠母猪接种疫苗后对仔猪免疫功能的影响。选取妊娠60 d母猪随机分为A、B 2组，A组母猪接种HP PRRS减毒活疫苗（TJM-F92株），B组母猪接种等量生理盐水，2组所产仔猪于10、20、30日龄时检测PRRSV、猪瘟病毒（CSFV）、猪伪狂犬病病毒（PRV）母源抗体，以MTT法测定外周血T、B淋巴细胞转化率、以琼脂平板法测定血清溶菌酶含量、以Griess试剂法测定NO含量。试验结果表明，妊娠母猪免疫HP-PRRS减毒活疫苗（TJM～F92株）后，其所产10日龄仔猪PRRSV母源抗体水平显著高于对照组仔猪（P〈0．05），仔猪CSFV阻断抗体极显著增高（P〈0．01），20日龄仔猪PRV感染抗体显著下降（P〈0．05）；免疫组和对照组所产仔猪的T、B淋巴细胞转化率及NO、溶菌酶含量均无显著差异（P〉0．05）。提示，妊娠母猪免疫HP-PRRS减毒活疫苗（TJM-F92株），对所产仔猪非特异性免疫功能和T、B免疫功能均无免疫抑制作用，仔猪抗PRRSV母源抗体、抗CSFV阻断抗体均显著增高且PRV感染抗体降低。

英文摘要：

To investigate the impact of vaccination with highly pathogenic porcine reproductive and respiratory syndrome （HP-PRRS） attenuated vaccine （TJM-F92 strain） at gestation of 60 days on immune competency for their piglets. The field test was performed using sows vaccinated with HP-PRRS attenuated vaccine （TJM-F92 strain） as the immunized group, and the unimmunized sows were injected with saline under the same condition as control. The serum and anti-coagulated blood was taken randomly from their piglets at days 10, 20 and 30, for detection of maternal antibodies against PRRSV, CSFV, PRV by ELISA, and T, B lymphocyte function by MTT assay, NO level by Griess＇s method and lysozyme activity by agar plate meth- od. The results showed that the vaccination with HP-PRRS attenuated vaccine （TJM-F92 strain） on the sows at gestation of 60 daysenhanced the immunologic defense ability for their piglets against PRRSV, the maternal antibody against PRRSV was signifi cantly higher than that of control group at day 10 （P〈0. 05）, and it was benefit for the ability of anti-CSFV and anti-PRV abilities, the blocking antibody against CSFV at day 10 was extremely significantly higher and the infectious antibody against PRV （gE） at day 20 was extremely significantly lower compared with the control group （P〈0.01）. The results also showed no sig- nificant immunosuppression on T, B lymphocyte transformation assay, NO level and lysozyme content in serum from the piglets between two groups （P〈0. 05）. The results indicated that the vaccination with HP-PRRS attenuated live vaccine （TJM-F92 strain） at gestation increased maternal antibodies against PRRSV and CSFV, whereas no suppression on the non-specific immu- nity and T, B cell immunity in their piglets.