中文摘要：

适合真梅系梅花品种‘铁骨红’生长的最佳培养基是改良Q＆L培养基，即Quoirin and Lepoivre培养基大量元素＋Perez培养基微量元素＋MS有机物＋2倍MS铁盐＋4mg·L^-1AgNO3（注意AgNO3长时间使用会导致试管苗生长畸形），最佳的增殖培养基是改良Q＆L培养基＋1.0mg·L^-16-BA＋0.05mg·L^-1NAA＋0.5mg·L^-1GA3；最佳的生根培养基是1/2改良Q＆L培养基＋0.3mg·L^-1NAA＋0.1mg·L^-1IBA。

英文摘要：

The optimum in vitro shoot section explants growing medium for Prunus mume cultivar Tiegu Hong was the modified Quoirin and Lepoivre medium supplemented with Quoirin and Lepoivre medium large elements, Perez medium trace elements, Murashige and Skoog medium （MS） organic compounds, and double MS Fe salt. This modified medium was used to resolve a serious of problems faced in Tiegu Hong tissue culture such as seedling chlorosis and shoot tip death. The optimum proliferation medium for Tiegu Hong was the im- proved Quoirin and Lepoivre medium supplemented with 1.0 mg·L^-1 6-BA, 0. 05 mg·L^-1 NAA and 4 mg·L^-1 AgNO3. Attention should be paid to the application of AgNO3 because of long period of use may lead to the plantlets malformation. The optimum rooting medium for Tiegu Hong was the improved 1/2 Quoirin and Lepoivre medium supplemented with 0. 3mg·L^-1 NAA and 0. 1 mg·L^-1 IBA.