中文摘要：

目的：对不同周龄的Fmr1 （ fragile X mental retardation 1 ）基因敲除和野生型雄性小鼠睾丸组织诱导型一氧化氮合酶（ inducible nitric oxide synthase,iNOS）的表达进行分析比较，探讨，-mrl基因敲除小鼠睾丸组织中iNOS表达的差异，为脆性x综合征的研究提供背景资料。方法：不同周龄（4、6、8、10周）的Fmr1基因敲除型和野生型雄性小鼠各6只，先采用PCR技术对基因敲除和野生型小鼠进行基因型鉴定，之后所有小鼠麻醉取睾丸组织，采用石蜡包埋切片免疫组化染色技术对基因敲除和野生型小鼠睾丸iNOS的表达进行检测并作对比分析。结果：iNOS在4周野生型小鼠睾丸间质细胞呈弱阳性表达，在6周小鼠呈阳性表达，在8周和10周小鼠呈强阳性表达，且基因敲除小鼠睾丸的iNOS表达均弱于野生型小鼠。结论：砌r1基因敲除小鼠在缺失FMR1蛋白（FMRP）后的睾丸组织中iNOS的表达降低。

英文摘要：

Objective： To analyze the expression of inducible nitric oxide synthase （iNOS） in the testis tissues of Frnrl （fragile X mental retardation 1 ） knockout and wild-type male mice in different developmental stages, and provide background information for researches on fragile X syndrome. Methods ： This study included 4, 6, 8 and 10 weeks old Fmrl knockout and wild-type male mice, 6 in each age group. We identified the genotype of the mice by PCR, and detected and compared the expression of iNOS in the testis tissues of the Fmrl knockout and wild-type mice by immunohistochemistry. Results ： The iNOS expression was weakly positive in the Leydig cells of the 4-week-old mice, moderately positive in the 6-week-old ones, and strongly positive in 8- and 10-week-old ones, sig- nificantly weaker in the Fmrl knockout than in the wild-type ones. Conclusion ： The expression of iNOS significantly decreases in the testis of Fmrl knockout mice, suggesting that iNOS may be involved in the pathogenesis of fragile X syndrome.