中文摘要：

目的：研究肿瘤坏死因子-α（TNF-α）对Caco-2细胞表达多聚免疫球蛋白受体（pIgR）和核因子-κB（NF-κB）亚单位RelA/RelB的影响。方法：采用Real-time PCR方法检测肠上皮细胞表达pIgR、RelA和RelB的变化。结果：与无TNF-α刺激比较,用不同浓度TNF-α刺激后pIgR、RelA和RelB基因的表达明显增加（P〈0.01）。结论：TNF-α促进肠上皮细胞表达pIgR、NF-κB亚单位基因表达,TNF-α通过NF-κB通路激活pIgR基因的转录。

英文摘要：

Objective：To study the influence of tumor necrosis factor-α（TNF-α）on the expression of RIgR and RelA/RelB subunits of nuclear factor-kappa B（NF-κB）in Caco-2 cells.Methods：pIgR mRNA,RelA and RelB mRNA of Caco-2 cells were assayed by quantitative Real-time PCR.Results：The increase of expression of genes,RelA and RelB gene in Caco-2 cells was found by the stimulation of TNF-α treatment compared with that of untreatment（P〈0.01）.Conclusion：TNF-α stimulates the expression of RIgR genes,RelA/RelB genes in Caco-2 cells.TNF-α may activate transcription of the PIgR gene through the alternative NF-κB pathway.