中文摘要：

以构建的表达拟南芥热激因子HsfA1a C端氨基酸331到氨基酸486（简称△HsfA1a）的大肠杆菌Ecoli M15为材料，用异丙基硫代-β-D-半乳糖苷（IVFG）诱导表达△HsfA1a，再通过Ni—NTA—Agarose亲和层析纯化表达的△HsfA1a，通过SDS—PAGE电泳分析表达蛋白和纯化蛋白，获得了表达纯化的△HsfA1a．

英文摘要：

E coli M15 was used as experimental materials. Expression of △HsfA1a in Arabidopsis thaliana was induced with isopropyl - β - D - galactoside （ IPTG）. △HsfA1a was purified by the Ni - NTA - Agarose affinity chromatography. △HsfA1a was analyzed by the SDS - PAGE electrophoresis. Heat shock factor △HsfA1a in Arabidopsis thaliana was expressed and purified. Experimental material was provided for isolating and identifying of heat shock elements （HSE） bound in vivo by heat shock factor HSF in Arabidopsis thaliana.