中文摘要：

目的 建立人结直肠癌裸鼠原位移植动物模型,并对其生物学特性进行检测,为探讨人结直肠癌的治疗提供实验工具.方法 应用外科手术裸鼠盲肠浆膜下原位移植的方法建立人结直肠癌裸鼠原位移植动物模型,并通过免疫组化法检测其生物学特性.结果 原位移植动物模型的成功率为100％,成功建立了人结直肠癌裸鼠原位移植模型,免疫组化检测发现肿瘤组织中增生细胞核抗原及Survivin高表达,该模型很好地模拟了人结直肠癌的生物学特征.结论 人结直肠癌裸鼠原位移植动物模型的建立为研究结直肠癌发生发展、转移机制及治疗提供了理想的实验动物模型.

英文摘要：

Objective To establish and characterize human colorectal cancer orthotopic implantation model in nude mice.Methods Established the human colorectal cancer orthotopic implantation model by surgery,characterize its biological characteristics by immunohistochemistry.Results Human colorectal cancer orthotopic implantation model was established in nude mice successfully,the proliferating cell nuclear antigen and Survivin were overexpressed in the models which were simulated the characteristics of human colorectal cancer perfectly.Conclusion This human colorectal cancer orthotopic implantaion model will provide an excellent animal model for us to study the development and metastasis mechanism of human colorectal cancer.