中文摘要：

通过阴离子交换层析和凝胶过滤层析，从黄鳝血清中得到其血清白蛋白，命名为MAA—semm，分子量为67kDa，为单链非糖蛋白，其N末端氨基酸序列为GHKKW。用0．5mg／只MAA—serum作为免疫源免疫大白兔，经3次免疫后，抽取大白兔血液，分离血清，饱和硫酸氨沉淀，获得抗MAA—semm的多克隆抗体。经ELISA方法测定该抗体与黄鳝血清白蛋白的免疫反应，抗体滴度为l：100000。经初步研究发现黄鳝皮肤中也有大量血清白蛋白分布，该抗体为进一步研究MAA-serum在皮肤中的分布及生物学功能打下基础。

英文摘要：

Serum albumin of Monopterus albus was obtained through the steps of mixing ion-exchange and gel filtration chromatography, and named MAA-serum, which is a single chain protein with molecular weigh of 67 kDa. MAA-serum is not a glycoprotein. The N-terminal amino acid sequence is GHKKW. The purified MAA-serum was used to immunize rabbits to prepare polyclonal antibody with the dosage of 0.5 mg/per rabbit. After immunized three times, the rabbits were anesthetized with ether, then the blood was collected by a cardiac puncture, and precipitate with saturated ammonium sulfate to obtain polyclonal antibody. ELISA data demonstrated that the antibody titer of the serum was up to 1：100 000. The anti MAA-serum polyclonal antibody had a high quality of potency and specificity. The antibody provided an useful experimental tool to profoundly research the tissue expression profile and biological function of MAA-serum.