中文摘要：

目的检测阿尔泰瑞香可能含有的化学成分,测定阿尔泰瑞香总酚含量,研究阿尔泰瑞香对食管癌Eca109细胞凋亡的影响。方法采用不同化学定性方法对阿尔泰瑞香中化学成分进行研究;以没食子酸为对照品,采用福林酚比色法测定了总酚含量;以Annexin V-FITC/PI双标记流式细胞术检测肿瘤细胞凋亡情况。结果阿尔泰瑞香含有黄酮、香豆素、糖、生物碱、酚等化学成分。阿尔泰瑞香乙醇提取物（DA-Et）中总酚含量为159.78 mg/g,测定方法平均回收率为99.4%-108.3%,精密度相对标准偏差为2.04%。DA-Et处理的食管癌细胞凋亡率为（29.633±1.779）%。结论本研究为阿尔泰瑞香质量标准的提升与合理应用提供了参考,为深入研究阿尔泰瑞香的活性成分提供了依据。

英文摘要：

Objective To investigate the chemical constituents of the ethanol extract of Daphne altaica Pall（DA-Et）,to determine total phenolic content in DA-Et and to study the effect of DA-Et on cell apoptosis in Eca-109 cells. Methods Different chemical assays were performed to detect chemical constituents of DA-Et. The content of total phenolics,ex-pressed as gallic acid equivalents,was measured by Folin- Ciocalteu assay; Cell apoptosis induced by DA-Et was detectedby Annexin V-FITC/PI flow cytometry. Results Flavonoids,coumarins,sugars,alkaloids and phenolics in DA-Et. Thecontents of total phenolics in DA- Et was quantified as 159.78 mg/g. Average recovery rate of this method ranged from99.4% to 108.3% and the precision relative standard deviation was 2.04%. Apoptosis rate of Esophageal cancer cell lineEca-109 induced by DA-Et was detected as 29.633%±1.779%（n=3）. Conclusion Our study provide guidance for the improvement of DA-Et quality, its appropriate application and further research of its activity.