中文摘要：

目的探讨低氧培养对大鼠骨髓间充质干细胞（MSCs）增殖、粘附及分泌的影响。方法通过细胞增殖实验、细胞粘附实验及Western—blotting，分别观察大鼠MSCs在低氧（6％O2）和常氧（21％O2）的增殖、粘附，及纤连蛋白（FN）和玻璃粘连蛋白（VN）的表达。结果低氧组10d内培养即可扩增到（1．3±0．12）×10^5细胞／孔，明显高于常氧组（（0．68±0．06）×10^5）细胞／孔（P〈0．05）；低氧组24h细胞粘附率（（89．14±2．4）％）高于常氧组（（62．13±2．6）％）（P〈0．05），同时FN和VN的蛋白表达显著增加（P〈0．05）。结论低氧作为体外的一种可控制因素，调节MSCs的增殖及粘附功能，对于MSCs的组织工程的应用具有一定的意义。

英文摘要：

Objective To investigate the effects of low oxygen tension on the proliferation,adhesion and secretion of mouse mesenchymal stem cells （MSCs）. Methods Proliferation test, adhesion assay and western-blotting were performed to observe proliferation, adhesion, and the protein expression of fibronectin and vitroneetin of mouse MSCs ,which cultured in 6%O2 and 21%O2. Results Compared to 21%O2, （1.3 ± 0.12） × 10^5 cells/well could be obtained after 10 days culture in 6%O2 （P〈 0.05）. Ration of 24h adhesion in 6%02 group （ （89.14 ± 2.4）% ） were signieantly higher than that in 21%O2 group（ （62.13 ± 2.6）% ）（ P 〈 0.05 ）. The protein expression of FN and VN were also higher in 6%O2 group（P 〈 0.05 ）. Conclusion Low oxygen tension as a vitro factor which can be controlled in vitro can regulate MSCs in proliferation, adhesion and extracellular matrix secretion, and have certain significance in the applying of MSCs in tissue engineering.