中文摘要：

[Objective] The paper aimed to establish a real-time fluorescent quantitative PCR( qPCR) detection method for Pineapple mealybug wilt associated virus-3( PMWa V3). [Method] Specific TaqMan probe and primers were designed and synthesized according to the conserved sequence of coat protein( CP) gene of PMWa V-3,and the standard curve was established after optimizing the amplification condition of qPCR. [Result]The results showed that the method was specific for the detection of PMWa V-3,and the sensitivity of the present method was about 10 times higher compared to general RT-PCR; the variation coefficients of intraassay and inter-assay were less than 1. 73,respectively. [Conclusion] The qPCR is an easy,fast and reliable method for quantitative detection of PMWa V-3.

英文摘要：

[ Objective ] The paper aimed to establish a real-time fluorescent quantitative PCR （qPCR） detection method for Pineapple mealybug wilt associated vi- rus-3 （ PMWaV3 ）. [ Method] Specific TaqMan probe and primers were designed and synthesized according to the conserved sequence of coat protein（CP） gene of PMWaV-3, and the standard curve was established after optimizing the amplification condition of qPCR. [ Result] The results showed that the method was specific for the detection of PMWaV-3, and the sensitivity of the present method was about 10 times higher compared to general RT-PCR ; the variation coefficients of intra- assay and inter-assay were less than 1.73, respectively. [ Conclusion] The qPCR is an easy, fast and reliable method for quantitative detection of PMWaV-3.