中文摘要：

小脑的 fastigial 原子核(FN ) 在马达控制和身体平衡保持一个重要角色。以前的研究表明了原子核被 directhypothalamocerebellar histaminergic 纤维刺激。然而，在小脑的 FN 的 histaminergic 设计的功能的角色从来没被建立过。在这研究，我们在由使用片准备小脑的 FN 开火的 neuronal 上调查了组织安的效果。65 个 FN 房间从 47 小脑的片被记录，并且房间的一个广阔多数与有刺激性的回答对组织安刺激作出回应(58/65， 89.2%) 。有媒介没堵住的 Iow-Ca~(2+)/high-Mg~(2+) 的酒的片导致组织安的刺激(n = 10 ) ，在房间上支持组织安的一个直接 postsynaptic 行动。而且， FN 神经原上的组织安的有刺激性的效果没被选择组织安 H_1 受体对手 triprolidine 堵住(n = 15 ) 或扑尔敏(n= 10 ) ，但是被 ranitidine 有效地压制(n = 15 ) ，一个高度选择的组织安 H_2receptor 对手。在另一方面，高度选择的组织安 H_2 受体收缩筋 dimaprit (n= 20 ) 而不是组织安 H_1 受体收缩筋 2-pyridylethylamine (n = 16 ) 在 FN 神经原上模仿了组织安的有刺激性的效果。导致 dimaprit 的 FN neuronal 刺激被选择组织安 H_2 受体对手 ranitidine 有效地反对(n = 13 ) 然而并非由选择组织安 H_1 受体对手 triprolidine 影响了(n = 15 ) 。这些结果证明组织安经由组织安 H_2 受体机制使小脑的 FN 房间激动并且建议 thehypothalamocerebellar histaminergic 纤维可以在 FN 神经原上通过他们的有刺激性的神经分布调制小脑的调停 FN 的感觉运动的集成。

英文摘要：

The cerebellar fastigial nucleus （FN） holds an important role in motor control and body balance. Previous studies have revealed that the nucleus is innervated by direct hypothalamocerebellar hletaminergic fibers. However, the functional role of histaminergic projection in cerebellar FN has never been established. In this study, we investigated the effect of histamine on neuronal firing of cerebellar FN by using slice preparations. Sixty-five FN cells were recorded from 47 cerebellar slices, and a vast majority of the cells responded to histamine stimulation with an excitatory response （58/65, 89.2%）. Perfusing slices with low-Ca^2＋/high-Mg^2＋ medium did not block the histamine-induced excitation （n=10）, supporting a direct postsynaptic action of histamine on the cells. Furthermore, the excitatory effect of histamine on FN neurons was not blocked by selective histamine H1 receptor antagonist triprolidine （n=15） or chlorpheniramine （n=10）, but was effectively suppressed by ranitidine （n=15）, a highly selective histamine H2 receptor antagonist. On the other hand, highly selective histamine H2 receptor agonist dimaprit （n=20） instead of histamine HI receptor agonist 2-pyridylethylamine （n=16） mimicked the excitatory effect of histamine on FN neurons. The dimaprit-induced FN neuronal excitation was effectively antagonized by selective histamine H2 receptor antagonist ranitidine （n=13） but not influenced by selective histamine H1 receptor antagonist triprolidine （n=15）. These results demonstrate that histamine excites cerebellar FN cells via the histamine H2 receptor mechanism and suggest that the hypothalamocerebellar histaminergic fibers may modulate cerebellar FN-mediated sensorimotor integration through their excitatory innervations on FN neurons.