中文摘要：

为了探讨脉冲电场（PEF）和X射线联合照射对小鼠雄性生殖和造血器官的复合效应,将74只成年雄性昆明种小鼠随机分成4组,即对照组、PEF组、X射线组以及PEF＋X射线复合组。将PEF辐照参数设定为电场强度1 k V/m和重复频率10 k Hz。动物每天接受PEF辐照1次,每次辐照时间为10 s,连续辐照3 d。PEF辐照结束24 h后复合组动物接受X射线全身照射,吸收剂量为7.0 Gy,吸收剂量率为1.1 Gy/min。照射完毕,观察动物体质量、外周血常规指标,睾丸、附睾、脾脏和胸腺脏器系数及睾丸形态结构,脾结节计数,动物15 d存活率。研究结果表明：与对照组相比,PEF组动物体质量、外周血常规指标、睾丸等脏器系数变化不显著,X射线组和复合组动物体质量明显降低,白细胞、淋巴细胞、血小板数量明显减少,胸腺和脾脏系数明显降低,睾丸组织结构损伤明显。与X射线组相比,复合组动物体质量、血液常规指标和脾结节数目变化不显著,但睾丸组织病理损伤明显减轻,动物15 d存活率有增高趋势。可得结论：单独脉冲电场对小鼠雄性生殖器官和造血器官无明显影响,并且对X射线引起的造血损伤无复合效应,但对X射线造成的睾丸组织损伤具有一定程度的保护作用。

英文摘要：

To investigate the effects of pulsed electric field（PEF） combined with X-ray on the reproduction and hematopoietic organs in male mice, we conducted experiments using 74 adult male mice, which were randomly divided into four groups, namely control group, PEF exposure group, X-ray group, and PEF ＋ X-ray group. The four groups were exposed to PEF with electric field intensity of 1 k V/m and repetition frequency of 10 k Hz. Each mouse was whole-body exposed to PEF once a day for 3 days, 10 seconds each time. 24 hours after the PEF exposure, the mice in PEF ＋ X-ray group were whole-body treated with X-ray with dose of 7.0 Gy at dose rate of 1.1 Gy/min. After the treatment, we measured several indexes of each mouse, including their weight, conventional indicators of peripheral blood, the organ indexes of testis, epididymis, spleen, and thymus, the morphology of testis, and the number of spleen nodule, and evaluated the 15-day survival rate of the treated mice as well. The experimental results show that, compared with the control group, the weight, conventional indicators of peripheral blood, organ indexes of mice in PEF group barely change, while the body weight, the number of leukocyte, lymphocytes and platelets, the coefficient of thymus and spleen of X-ray group and PEF ＋ X-ray group significantly decrease, and the structures of testicular tissue of these two groups are obviously damaged. Compared with the X-ray group, the PEF＋X-ray group show no obvious change in the body weight, the conventional in-——dicators of blood, and the number of splenic nodules, but the testicular histopathological damage is alleviated significantly and a 15-day survival rate tends to increase. It is concluded that PEF has neither obvious effect on reproductive organs and hematogenic organs in male mice, nor combined effect with X-rays, nevertheless, it can protect testicular tissue from X-ray induced damage to some extent.