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中文摘要：
目的探索SsuDAT1I限制修饰系统在不同背景的猪链球菌2型（SS2）分离株中的分布规律。方法选取29个不同背景SS2分离株，通过分析其全基因组序列或PCR检测purH和purD基因间是否含有SsuDAT1I插入序列，并分别用MobI和DpnI酶切SS2基因组以鉴定其5LGATC-3’序列位点是否存在甲基化。结果29株SS2中有6株携带SsuDAT1I限制修饰系统，且均为无（弱）毒株；其他23株不带SsuDATlI的SS2中有22株为强毒株，仅有1株为1980年代在荷兰分离的弱毒株T15。酶切显示，含有SsuDAT1I的SS2菌株的基因组DNA可被DpnI完全酶切，但不能被MobI所酶切；其他缺失SsuDAT1I的SS2菌株的酶切图谱则完全相反。证明SsuDAT1I系统可对SS2基因组的5LGATC-3’位点进行甲基化修饰。结论作为SS2基因组获得的外来遗传元件，对SsuDAT1I的鉴定有助于分析SS2菌株的系统进化关系和毒力强弱。
英文摘要：
Objective To examine the regular distribution of the SsuDAT1 I restriction-modification system in Strepto coccus suis 2 （SS2） isolates with different origins. Methods Twenty-nine strains of SS2 with different origins were investigated using PCR assay or whole genome sequence analysis to determine whether or not the SsuDAT1I gene was inserted in the genome between purH and purD. Methylation of the 5p GATC-3＇ sequence was determined by testing the susceptibility of genomic DNA to the restriction endonucleases Dpn I and Mob I in vitro. Results Results indicated that the SsuDATII system is present only in the 6 nonvirulent or weakly virulent strains out of the 29 SS2 strains exam ined in this study. Of the other 23 SS2 strains, 22 were highly virulent, and one Netherlands strain, T15, was avirulent. Genomic DNA isolated from SsuDATII phenotype strains was digested into small fragments by Dpn I but not by Mob I . In contrast, DNA from the strains with the null phenotype lacked SsuDAT1I and was digested only by Mob I . This indicates that the 5＇-GATC-3＇ sequence in the DNA of these SS2 strains was methylated by the SsuDAT1I system. Conclusion These results suggest that SsuDAT1I may serve as a useful marker in analyzing the phylogenetic relationship and predicting the pathogenic behavior of SS2 strains.
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