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中文摘要：
目的：以复合蛋白酶与风味蛋白酶双酶解的马鹿角蛋白酶解物和马鹿角蛋白作为受试样品,研究样品的抗氧化、抗疲劳和免疫调节功能。方法：采用邻苯三酚自氧化法、水杨酸比色法和总还原力测定法,研究酶解物体外抗氧化的功能;通过测定酶解物灌胃小鼠的血清中超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量,分析其体内抗氧化功能;通过小鼠负重游泳、血乳酸、肝糖原测定研究酶解物的抗疲劳功能;通过ConA诱导的小鼠脾淋巴细胞转化实验及小鼠碳廓清实验研究酶解物对小鼠免疫功能的影响。结果：马鹿角蛋白酶解物对羟自由基（·OH）、超氧阴离子自由基（O2-.）均有清除能力,且高于同等质量浓度的马鹿角蛋白,总还原力与马鹿角蛋白相比也有提高;可提高小鼠血清SOD活性,降低其血清MDA含量,并明显延长小鼠负重游泳时间、降低血乳酸值和增加肝糖原含量;可显著增强小鼠的免疫功能。结论：马鹿角蛋白酶解物具有抗氧化、抗疲劳和提高免疫功能的作用,活性明显优于马鹿角蛋白。
英文摘要：
The antioxidant,anti-fatigue and immuoregulatory effects of red deer（Cervus elaphus） antler protein and hydrolysate prepared with both protamex and flavourzyme were investigated.For the determination of antioxidant effects,pyrogallol auto-oxidation method,salicylate colorimetry and total reducing power assay were used.The in vivo antioxidant potential of antler protein hydrolysate was analyzed by measuring SOD activity and MDA content in the serum of mice receiving oral administration.Weight-loaded swimming test and blood lactic acid and liver glycogen assays were performed to assess its anti-fatigue effect.Besides,the immunoregulatory effect of the hydrolysate in mice was examined by ConAinduced mice spleen lymphocyte transformation and carbon clearance tests.Our results indicated that red deer antler protein hydrolysate possessed scavenging activity against hydroxyl and superoxide anion radicals and improved total reducing power compared to red deer antler protein.In addition,oral administration of this hydrolysate increased serum SOD activity,reduced serum MDA content,obviously prolonged swimming time,decreased blood lactic acid and liver glycogen levels,and remarkably enhanced the immune function of mice.From these observations,we concluded that red deer antler protein hydrolysate has significantly stronger antioxidant,anti-fatigue and immuoregulatory effects than red deer antler protein.
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