中文摘要：

研究了时间分辨荧光标记与检测组合式DNA芯片的方法。首先在薄玻璃片上原位合成寡核苷酸的序列片段，然后将之裁成小片，随后将带有不同DNA片段序列的小片拼接组合成组合式DNA芯片。利用4，7-二氯磺酰苯基．1，10-菲罗啉-2，9-二羧酸（BCPDA）多重标记的亲和素-生物素放大效应，建立了时间分辨荧光检测组合式DNA芯片的方法：芯片上的DNA序列经过杂交后，其互补序列末端的生物素可将多重BCPDA标记的亲和素联接，然后由BCPDA对铕离子（Eu^3＋）捕获与解离来实现杂交信号差别，并可实现对正配、单碱基错配、二及三碱基错配的时间分辨荧光信号差别的检测。对BCPDA标记的系列化合物进行了荧光性能表征，并对时间分辨荧光检测与传统荧光检测模式作了比较。

英文摘要：

Utilizing 4, 7-bis （chlorosulfophenyl）-1, 10-phenanthroline-2, 9-dicarboxylic acid （BCPDA, abbreviated as BCPDA） labeling method based on avidin-biotin amplification, we established a TRF detection format on the mosaic DNA chip： the detection method allows discriminatory signals for perfect match, one-base mismatch, two-base mismatch and three-base mismatch by TRF labeled DNA hybridization, whereby Europium （Ⅲ, Eu^3＋） was captured and released on the principle of complexation and dissociation interaction between BCPDA and Eu^3＋ solution when the BCPDA-tagged avidin and biotin-capped oligonucleotide sequence linked. The fluorescence spectra and related lifetimes were determined. We also compared the TRF detection mode with the conventional fluorescence one.