中文摘要：

研究利用造孔剂法制备高度贯通的多孔羟基磷灰石（HA）支架,孔隙率约为78%,并利用聚己内酯（PCL）分别复合纳米HA（nHA）或微纳米生物玻璃（nBG）粉末对其进行涂覆改性,粉末的添加量均为10%-40%（质量分数）.4种类型支架分别记为HA、PCL/HA、nHA-PCL/HA和nBG-PCL/HA.实验结果发现,nHA-PCL/HA和nBG-PCL/HA复合支架最大抗压强度分别为1.41-1.98MPa和1.35-1.78MPa.4类支架矿化实验显示,浸泡21d后nBG-PCL/HA表面促进生成较多的磷灰石矿化物;细胞实验结果显示细胞在4类支架上均生长良好,说明支架具有良好的生物相容性.支架在实验犬背部肌肉组织内植入2个月的组织学检测显示,4种支架内均有新骨形成,尤其是nHA-PCL/HA和nBG-PCL/HA孔内有更多的新生骨组织,说明这两种支架表面复合涂层中的生物活性纳米颗粒对诱导新骨生成具有积极的促进作用.

英文摘要：

Highly interconnected porous hydroxyapatite (HA)scaffolds with high porosity (≈78%)were pre-pared by particle leaching method in the present work,and subsequently the as-prepared HA scaffolds were lined with PCL/nano-HA (nHA)or PCL/micro-nano-bioglass (nBG)composite coatings,the contents of the powder were varied from 10% to 40wt%.The four types of scaffolds were signed as HA,PCL/HA,nHA-PCL/HA and nBG-PCL/HA.The mechanical testing results showed that compressive strengths of nHA-PCL/HA and nBG-PCL/HA were 1.41-1.98 MPa and 1.35-1.78 MPa,respectively.The biomineralization results indicated that much apatite was formed on the surface of nBG-PCL coated scaffolds after immersed in SBF for 2 1 d.Cell culture experimental results showed that osteoblasts grew well on all scaffolds,indicating that all scaffolds possessed positive biological compatibility.Histological section evaluation showed that there was a large amount of new bone forming in nHA-PCL/HA and nBG-PCL/HA scaffolds comparing to other two scaf-folds after implanted for 2 months in the back muscle tissue of dogs,which exhibited that the two biological particles in composite coatings played a positive role in the induction of new bone formation at non-osseous sites.