中文摘要：

本试验旨在研究妊娠后期营养限饲对蒙古绵羊胎儿肝脏生长发育及抗氧化能力的影响。选择健康的蒙古绵羊42只（经同期发情受孕）,在妊娠90d时选择6只母羊进行屠宰,其余按体重随机分配到3个组：限制组1（0.175MJ ME·kgw-0.75·d-1,n=14,RG1）、限制组2（0.33MJ ME·kgw-0.75·d-1,n=12,RG2）和自由采食组（0.67MJ ME·kgw-0.75·d-1,n=10,CG）,进行不同能量水平饲养。饲喂至妊娠140d,各组再选择6只母羊进行屠宰。结果表明,RG1组胎儿重（P〈0.01）、胎儿肝脏重（P〈0.01）、肝脏中DNA浓度（P〈0.01）、蛋白浓度（P〈0.01）、总DNA含量（P〈0.01）、蛋白质/DNA（P〈0.01）、总抗氧化能力（P〈0.01）、超氧化物歧化酶活性（P〈0.05）显著低于CG组,而谷胱甘肽过氧化物酶活性（P〈0.05）和丙二醛浓度（P〈0.01）显著高于CG组;RG2组胎儿重（P〈0.01）、肝脏重（P〈0.05）、肝脏中DNA浓度（P〈0.01）、蛋白浓度（P〈0.01）、总DNA含量（P〈0.01）、蛋白质/DNA（P〈0.01）、总抗氧化能力（P〈0.01）显著低于CG组,而丙二醛浓度（P〈0.01）显著高于CG组。结果显示,妊娠后期营养限饲蒙古绵羊严重影响了其胎儿肝脏的生长发育和抗氧化能力,而且随着营养水平的降低,发育受阻程度加深,抗氧化防御敏感性增强。

英文摘要：

This study was conducted to investigate effects of maternal undernutrition during late pregnancy on growth,development and anti-oxidation capability of Mongolia ovine fetal liver.42 Mongolia ewes treated by estrus synchronization fecundability were selected,6 ewes were slaughtered at the beginning of the experiment and the remaining 36 animals were allocated to three different groups： restricted group1（RG1,0.175 MJ ME·kgw-0.75·d-1,n=14）,restricted group2（RG2,0.33 MJ ME·kgw-0.75·d-1,n=12） and control group（CG,ad libitum,0.67 MJ ME·kgw-0.75·d-1,n=10）.At 140 d of gestation,6 representative ewes in each group were slaughtered.The results showed as follows： fetal weight（P0.01）,fetal liver weight（P0.01）,DNA concentration（P0.01）,protein concentration（P0.01）,total DNA contents（P0.01）,protein/DNA（P0.01）,T-AOC（P0.01）,SOD activity（P0.05） in fetal liver were significantly decreased and GSH-PX activity（P0.05）,MDA concentration（P0.01） were significantly enhanced in RG1 group compared with CG group.For RG2 group,the decreases of fetal weight（P0.01）,liver weight（P0.05）,DNA concentration（P0.01）,protein concentration（P0.01）,total DNA contents（P0.01）,protein/DNA（P0.01）,T-AOC（P0.01） were founded and MDA concentration was significantly increased inRG2 group than that in CG group（P0.01）.In conclusion,the growth,development and anti-oxidation capability of fetal livel were affected seriously by maternal undernutrition during late pregnancy.With the decreasing of nutrition levels,the negative reactions to fetal liver became more severe and the sensitivity of anti-oxidation defense was increased.