中文摘要：

目的建立少数民族药大复方全组分的快速制备方法,为有效成分的高通量筛选提供大量样品。方法应用快速色谱仪,以蒙药白脉散乙酸乙酯提取部位为研究对象,采用线性梯度洗脱法快速划段制备一系列色谱组分,采用高效液相色谱法对分离效果进行评价。结果从该复方乙酸乙酯部位获得29个色谱组分,该方法对组方中不同性质的物质起到了较好的分离与富集效果。结论快速色谱技术可用于构建民族复方药物组分库,这将有利于攻克民族药大复方药效物质基础研究难题。

英文摘要：

Objective To establish the rapid method of multi-component library preparation of large ethnodrug compound prescription and offer large amounts of samples for active components high-throughput screening.Methods The ethyl acetate extract from Mongolian medicine BaiMai Powder was separated into a series of fractions by linear gradient elution with flash chromatography.The separation efficiency was evaluated by high-performance liquid chromatography.Results Twenty-nine sequential components were prepared from the ethyl acetate extract of the prescription,enrichment and separation of compounds with different nature were well complished.Conclusion Multi-component library of ethnodrug compound prescription can be constructed by flash chromatography.This method will help to overcome the difficulty of the pharmacodynamic chemical substances research of large ethnodrug compound prescription.