中文摘要：

以拟南芥野生型和突变体Athspr为材料，采用双向电泳技术对其在0h（对照）、2h及8h热激条件下的叶片全蛋白进行了分离。应用ImageMaster软件从以上蛋白电泳图谱中分别鉴定出1000多个蛋白点，发现其蛋白表达谱在野生型和突变体中存在明显差异。同时选取在野生型和突变体中表达明显有差异的3个高丰度蛋白质点进行了质谱鉴定，并进一步通过GPS-MASCOT进行了数据库检索，鉴定出这三个蛋白质组分分别为富含甘氨酸的RNA结合蛋白7、细胞色素b6-f复合物铁硫亚单位及放氧增强蛋白1-2。以上3个蛋白均与植株在胁迫条件下的抗性有关。推测由于T—DNA的插入使得突变体中Athspr基因的功能受到了影响，进而影响了其下游其他基因的表达和上述3种蛋白的表达与功能，产生了突变体的耐热能力以及其他逆境条件下抗逆性下降的一系列表型改变。

英文摘要：

Total proteins from leaves of Arabidopsis wild type and mutant Athspr, subjected to heat shock for 0, 2 and 8 h, were isolated, using optimized methods of two-dimensional gel electrophoresis. The maps were analyzed with solftware ImageMaster, and more than 1000 spots were detected respectively, and exhibited obvious differences between mutant and wild type. The molecular weights of most proteins were distributed in 14.1-116.0kDa, and their isoelectric points were around pH 5-7. Three abundant differentially expressed protein spots between mutant and wild type plants were cut off, digested in gel with Trypsin, identified with MALDI-TOF-TOF-MS. Full amino acid sequences of proteins were obtained and identified through searching database with GPS-MASCOT. One of them is Glycine-rich RNA-binding protein 7 （GRP 7） , the other is Cytochrome b6-f complex iron-sulfur subunit, and the third is Oxygen-evolving enhancer protein 1-2, （OEE1）. All of them were related with plant resistance to stress, suggesting that T-DNA insertion could influence the functional activity of the gene in the mutant, alter the expressions and functions of those three proteins, and then lead to the alterations of phenotypes including decline of resistance to stress in the mutant.