中文摘要：

为了解植物miR159家族成员的分子特性及其进化规律,该研究对miRBase数据库中登录的miR159家族成员进行分类统计、进化树构建、科间比较、二级结构预测及靶基因分析.结果表明：miR159家族在植物界分布非常广泛,蕨类植物可能是miR159家族的进化祖先;系统发育进化树分析显示,植物miR159家族成员间存在多个进化分支,且进化关系与植物属性有关,即植物亲缘关系越近的成员更易成枝,且具有相同进化方向的成员序列高度同源;Mfold预测显示,pre-miR159均会自发形成典型、稳定的茎环二级结构,并包含19~21个碱基为单位的功能片段,每个单位均有可能形成miR159成熟体;靶基因分析发现,miR159家族成员主要作用于MYB转录因子、转座因子和假定蛋白等,但在不同物种间及相同物种的不同成员间作用的靶标种类与靶基因ID数量均存在差异,尤其是miR159-3p与miR159-5p间的差异最为显著.

英文摘要：

In order to explore the molecular characteristics and evolution of the miR159 family members,we studied the miR159 family members registered in the miRBase database by distribution statistics,constructing the phylogenetic trees,comparing different families,and predicting their secondary structures and target genes.The results showed that the miR159 family was widely distributed in the plant community,and the ferns may be the evolutionary ancestors of the miR159 family.Phylogenetic tree analysis showed that there were multiple evolutionary directions among the members of the plant miR159 family,but the miR159 family members of the same type of plants tended to be clustered,and the members from the same evolutionary direction were highly homologous.Mfold predicts that pre-miR159 spontaneously forms a typical,stable stem-loop secondary structure,and contains functional fragments ranged from 19~21 bases,which may be miR159 mature body.Target gene analysis showed that miR159 family members mainly regulate MYB transcription factor,transposable factor and hypothetical protein,but the types and numbers of target genes were differences among different species and among different members of the same species,especially between miR159-3p and miR159-5p.