中文摘要：

以菲为多环芳烃（PAHs）代表物,采用温室水培试验方法,研究了黑麦草、苏丹草、墨西哥玉米、高羊茅、三叶草等5种植物根亚细胞中菲的分配作用.结果表明,经144h培养,随着培养液中菲平衡浓度由0.056mg·L^-1增至0.39mg·L^-1,黑麦草根、细胞壁、细胞器中菲的含量分别从26.85、20.01和36.19mg·kg^-1增大到56.91、49.54和59.77mg·kg^-1,富集系数则分别由357.14、479.49和649.25L·kg^-1降低到145.92、127.04和153.26L·kg^-1.黑麦草根及亚细胞组分中菲的含量大小为细胞器〉根〉细胞壁,其中细胞器中菲含量要比细胞壁高21%～163%.水中菲的起始浓度均为1mg·L^-1时,144h后,供试5种植物根细胞器中菲的含量（48.64～145.2mg·kg^-1）均大于细胞壁（15.86～74.49mg·L^-1）.5种植物根亚细胞中菲分配的比例大小顺序为细胞器〉细胞壁〉可溶部分;其中,根内46%～53%和31%～40%的菲分别分布在细胞器和细胞壁中.

英文摘要：

Subcellular distribution of phenanthrene as a representative of polycyclic aromatic hydrocarbons （PAHs） in roots of five plants was investigated using a greenhouse hydroponic experiment. Results showed that,with the increase of the equilibrium concentrations of phenanthrene in solution from 0. 056 to 0. 39 mg·L ^-1,the respective concentrations of phenanthrene in root,root cell organelle and cell wall increased from 26. 85,20. 01 and 36. 19 mg·kg^-1 to 56. 91,49. 54 and 59. 77 mg·kg^-1,and their concentration factors decreased from 357. 14,479. 49,and 649. 25 L·kg^-1 to 145. 92,127. 04,and 153. 26 L·kg^-1,respectively. The concentrations of phenanthrene in ryegrass root tissues followed the order of cell organelle root cell wall. In addition, concentrations of phenanthrene in cell organelle were generally 21% -163% higher than those in cell wall. As to the roots of five different tested plants growing in solution with initial phennathrene concentration of 1 mg·L ^-1 after 144 h cultivation,the concentrations of phenanthrene were 48. 64 -145. 2 mg·kg^-1 in root cell organelle,which were observed to be higher than those （15. 86 -74. 49 mg·kg^-1） in root cell wall. The subcellular distribution （%） of phenanthrene was in the following order： cell organelle cell wall cell soluble fraction. Based on the detected amounts of phenanthrene in subcellular fractions of plant root tissues, about 46% -53% and 31% -40% of phenanthrene were observed in root cell organelle and cell wall.