在温室内用自动控制装置模拟正规半日潮淹浸作用对老鼠簕种子发芽与幼苗生长的影响,设淹水0、3、6、9、12、15、18、21和24h·d-1共9个淹水时间梯度,人工海水盐度为2‰,测定不同淹水时间下老鼠簕种子萌发、幼苗株高、叶面积、生物量以及幼苗叶脯氨酸含量、丙二醛(MDA)含量、叶绿素含量和超氧化物歧化酶(SOD)活性等生理生化指标。结果表明,长时间淹水(≥15h·d-1)会推迟老鼠簕种子萌发的时间,并使发芽率和成苗率降低,淹水〈15h·d-1适宜老鼠簕种子的萌发。随着淹水时间的增长,脯氨酸含量、MDA含量和SOD活性均先下降后上升,叶绿素含量则逐渐降低。淹水≥3h·d-1,幼苗生物量、单株叶面积、高生长和叶绿素含量与淹水时间均呈负相关。淹水0~3h·d-1,老鼠簕总叶绿素含量、根、茎、地上部分和总生物量积累均显著大于其他处理,淹水≥18h·d-1,老鼠簕幼苗总生物量、叶面积、叶绿素含量显著降低,根冠比开始上升。在上述人工培养条件下,淹水0~3h·d-1是老鼠簕幼苗生长的最佳淹水时间,18h·d-1是老鼠簕幼苗生长的一个临界淹水时间,淹水≥18h·d-1老鼠簕幼苗生长显著受到抑制。
By using automatically controlled device,a greenhouse mud culture experiment was conducted to study the effects of simulated regular semidiurnal tide inundation on the seed germination and seedling growth of mangrove species Acanthus ilicifolius.The inundation time gradient was installed as 0,3,6,9,12,15,18,21 and 24 h ·d-1,and the artificial seawater with a salinity of 2‰ was prepared.Some physiological and biochemical parameters,including seed germination rate,seedling height and biomass,leaf area,leaf proline,malonyldialdehyde(MDA),and chlorophyll contents,and leaf superoxide dismutase(SOD) activity were determined.For the A.ilicifolius,longer term(≥15 h·d-1) tide inundation delayed the seed germination and decreased the germination rate,while shorter term(〈15 h·d-1) inundation was beneficial to the seed germination.With increasing inundation duration,the leaf proline and MDA contents and SOD activity increased after an initial decrease,while the leaf chlorophyll content decreased gradually.Under ≥3 h·d-1 inundation,the seedling biomass,height growth,leaf area per plant,and leaf chlorophyll content all had a negative correlation with inundation duration.Under 0-3 h·d-1 inundation,the total biomass,dry weights of root,stem,and shoot,and leaf chlorophyll content were significantly higher than those in other treatments.In contrast,under ≥18 h·d-1 inundation,the total biomass,leaf area,and leaf chlorophyll content decreased significantly,and the root/shoot ratio increased gradually.In this study,0-3 h·d-1 was the optimal duration of inundation for the seedling growth of A.ilicifolius,and 18 h·d-1 was the critical duration.When the inundation duration was ≥18 h·d-1,the seedling growth was inhibited significantly.