中文摘要：

有机磷农药药效高、易于被水、酶和微生物所降解，残留毒性小，但由于不合理利用等因素，粮食、蔬菜、水果和环境中的有机磷农药残留严重超标，其危害引起了各国政府、学术界、民众的高度重视．目前有色谱法、酶法和免疫法等，这些方法的应用都受到客观因素的限制．因此研制类似于酶和抗体的高选择性、低造价、稳定性好的吸附材料用于食品和环境中有机磷农药残留的富集、分离纯化和分析尤为重要．

英文摘要：

Unit＇ormly imprinted polymer microspheres were prepared by precipitation polymerization in chloroform MCP （ monocrotophos ）, MAA （ methacrylic acid ）, EGDMA （ ethylene glycol dimethacrylate ） and AIBN （azobisisobutyronitrile） were used as template, functional monomer, cross-linker and initiator, respectively. UV and IR spectra were used to demonstrate the mechanism of the interaction between MCP and MAA . It was found that one MCP was entrapped by three MAA molecules in chloroform. Langmuir model was used to fit the adsorption data, the results showed that the MCP imprinted polymer microspheres showed specific binding sites to the MCP. The data obtained in equilibrium adsorption experiments were processed （ KDI ） and the apparent maximum binding capacity （ Bmaxl ） were by Scatchard analysis, the dissociation contents KD1= 0.062 mmol/L, Bmaxl = 3.26 μmol/g for high affinity binding sites and KD2= 3.03 mmol/L, Bmax2= 65.70 μmol/g for low affinity binding sites, respectively. The MCP imprinted polymers have showed higher affinity to MCP than to the other organophosphorus pesticide used. So the imprinted polymers microspheres can be used for trace pesticide residue separation, enrichment and analysis.