中文摘要：

化学感受蛋白（chemosensory proteins,CSPs）是昆虫体内存在的一类主要识别和运载非挥发性的气味分子和化学刺激物的可溶性蛋白。本研究运用半定量RT-PCR方法分析了BmCSP4的时空及组织表达谱。结果表明：BmCSP4在家蚕Bombyx mori各发育阶段均表达,但表达量从4龄到蛹期逐渐减少,且在雌成虫头部、胸部和腹部表达量较少。用1-NPN作为荧光探针,测定了15种外源配基与BmCSP4蛋白的结合特性,结果显示：仅芳香醛类和芳香酮类化合物在浓度10μmol/L能将1-NPN从BmCSP4中替换50%,苯甲醛解离常数为3.20μmol/L,对甲氧基苯甲醛解离常数为2.24μmol/L,2-戊基-3-苯丙基-烯醛解离常数为2.88μmol/L,1-苯基-1-丁酮解离常数为2.04μmol/L,苯乙酮解离常数为2.52μmol/L。据此推测,BmCSP4在不同的发育阶段执行不同的生理功能,并可能参与对芳香醛、芳香酮类气味识别过程。

英文摘要：

Chemosensory proteins（CSPs） are a class of small soluble proteins in insects,which are supposed to recognize and transport non-volatility odour molecules and chemical stimuli. In this study,we investigated the expression pattern of BmCSP4 using semi-quantitative RT-PCR. The results showed that BmCSP4 was expressed across various developmental stages,with gradual decrease from 4th larva to pupa. The tissue expression profile in males and females at adult stage was further studied,and the results showed that the expression level of BmCSP4 was lower in female head（with antennae removed） ,throax and abdomen than in other female tissues. The binding specificity of BmCSP4 to 15 compounds was tested using N-phenyl-1-naphthylamine（1-NPN） as fluorescent probe,and the results showed that only benzaldehyde,p-methoxy benzaldehy,2-pentyl-3-phenylpropenoic,1-phenyl-1-butanol and acetophenone at the concentration of 10 μmol/L replaced 1-NPN from BmCSP4 by 50%,with the dissociation constants of 3.20,2.24,2.88,2.04 and 2.52 μmol/L,respectively. These results suggest that BmCSP4 may have diverse physiological function in different developmental stages,and may be involved in the process of discriminating aromatic aldehyde and aromatic ketone compounds.