盐应力是禁止庄稼生长的一个主要环境因素。Trichoderma spp。是紧张的真菌和一些能刺激的最有效的 biocontrol 植物生长。当在支持的主要机制之一在咸度种生长,而是磷酸盐增溶的内在的机制,磷酸盐增溶被知道仍然需要被探索。在我们的实验室孤立并且识别的 Trichoderma asperellum Q1 是有一项高磷酸盐增溶活动的有益的根围 biocontrol 真菌。当把不可溶解的器官的磷用作唯一的磷来源,时,它能生产酸和碱的磷酸酶盐压力增加了紧张的磷增溶能力和二酶的活动。而且,酸磷酸酶被硫酸铵降水,离子交换,和胶化过滤层析从发酵肉汤净化。它的分子的重量是由 SDS 页决定了的 55 kDa。净化的酸磷酸酶被用来由板试金调查 Arabidopsis thaliana 的生长性能,结果证明它由在盐应力下面把器官的磷酸盐转变成一种可溶的无机的形式贡献了 Arabidopsis 生长。到我们的知识,这是从 T 的酸磷酸酶纯化上的第一份报告。在在盐应力下面的植物生长的规定的 asperellum 和它的功能。
Salt stress is a major environmental factor that inhibits crop growth.Trichoderma spp.are the most efficient biocontrol fungi and some of the strains can stimulate plant growth.Phosphate solubilization is known as one of the main mechanisms in promoting plant growth,but the underlying mechanisms of phosphate solubilization in the salinity still need to be explored.The Trichoderma asperellum Q1 isolated and identified in our lab is a beneficial rhizosphere biocontrol fungus with a high phosphate solubilization activity.It could produce acid and alkaline phosphatases when using insoluble organic phosphorus as the sole phosphorus source,the salt stress increased the phosphorus-solubilization ability of the strain and the activities of the two enzymes.Furthermore,an acid phosphatase was purified from the fermentation broth by ammonium sulphate precipitation,ion-exchange,and gel filtration chromatography.Its molecular weight was 55 k Da as determined by SDS-PAGE.The purified acid phosphatase was used to investigate growth performance of Arabidopsis thaliana by plate assay and the result showed that it contributed to Arabidopsis growth by transforming organic phosphate into a soluble inorganic form under salt stress.To our knowledge,this is the first report on acid phosphatase purification from T.asperellum and its function in regulation of plant growth under salt stress.