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大幅度提高马齿苋组培效率的培养环境条件优化

ISSN号：1005-4847
期刊名称：《中国实验动物学报》
时间：0
分类：Q647[生物学—生物物理学]
作者机构：[1]南京师范大学生命科学学院,江苏南京210023, [2]江苏省环洪泽湖生态农业生物技术重点实验室,淮阴师范学院,江苏淮安223300
相关基金：国家基础科学人才培养基金项目（J1103507）;国家基础科学人才培养基金项目（J1210025）; 江苏省环洪泽湖生态农业生物技术重点实验室开放课题（HZHL1504）; 江苏省大学生创新项目

作者：杨颜颜[1], 云晶晶[1], 杜建梅[1], 杨心怡[1], 王新风[2], 陆长梅[1]

关键词：马齿苋, 高温, 全光照, 愈伤组织诱导, 不定芽分化, Purslane, High temperature, Full light exposure, Callus induction, Bud differentiation

中文摘要：

为了提高马齿苋的组培效率,解决马齿苋出愈时间迟、愈伤组织诱导率不高、愈伤组织诱导需要使用2,4-D、不定芽增殖来源有限等问题,根据马齿苋的最适生长条件,将组织培养环境调整为温度（30±1）℃、全光照,并从无菌苗上直接取材进行外植体培养。结果表明：种子在高温、全光照条件下萌发快,且无菌苗长势好。取材于无菌苗上的根、茎和叶均可以在不添加2,4-D的MS培养基中脱分化形成愈伤组织,且茎在MS＋NAA 1.0 mg/L＋6-BA 1.0 mg/L培养基中、叶在MS＋NAA 1.5 mg/L＋6-BA 0.5-1.5 mg/L培养基中的初始出愈时间均提前至4 d,且诱导率均达到100%,愈伤组织长势旺盛。带腋芽的茎段在MS＋NAA 0.1 mg/L＋6-BA 5.0 mg/L培养基中可以直接分化出大量不定芽,增殖率达10.5。在最适光温环境下,马齿苋外植体出愈迅速,愈伤组织诱导率和不定芽增殖率大幅度提高。

英文摘要：

In order to improve the tissue culture efficiency of purslane,the problems,such as late callus formation, low rate of callus induction, requiring the use of 2,4-D in callus induction, and limited source of explants for adventitious buds proliferation etc. should be solved. According to the optimal growth conditions for purslane, its tissue culture environment was adjusted to（30 ±1）℃ of temperature and full light exposure, and explants were taken directly from the sterile seedlings. The results showed that seeds germinated well and aseptic seedlin gs grew fast under this condition. Callus could be induced from each organ of aseptic seedlings on MS＋NAA 1.0 mg/L＋6-BA 1.0 mg/L medium without the addition of2,4-D. Callus could be first found on the stems in 4 d and the callus grew very fast. After further adjust-ment for hormones,callus could be induced a hundred percent in 4 d from leaves on MS ＋NAA 1.5 mg/L＋6-BA 0.5-1.5 mg/L medium. A large number of adventitious buds could be induced from stems with axillary buds on MS＋NAA 0.1 mg/L＋6-BA 5.0 mg/L medium with a proliferation rate of 10.5. Under the optimum temperature and light environment, callus from purslane explants could be induced early. The callus grew fast, and its induction rate together with proliferation rate of adventitious buds were higher.