中文摘要：

目的探讨海水诱导的细胞死亡特点及其分子机制。方法海水与生理盐水按照不同比例稀释以及处理不同时间条件下，4’，6-二脒基-2-苯基吲哚（4’，6-diamidino-2-phenylindole，DAPI）染色并在荧光显微镜下观察血管内皮细胞株（endothelialcellofvesses，ECV）304细胞形态和生存改变，采用膜联蛋白V-异硫氰酸荧光素和碘化丙啶双染色结合流式细胞术观察ECV304细胞凋亡比例，采用实时定量聚合酶链式反应陈列芯片杂交分析凋亡相关基因表达水平的变化。结果海水刺激早期，ECV304细胞脱水皱缩，随时间延长，细胞凋亡与坏死逐渐增多。荧光显微镜和流式细胞术分析结果显示，海水：生理盐水=1：1稀释不仅导致细胞坏死，还能够诱导细胞凋亡，两者比例相当。实时定量聚合酶链反应陈列芯片杂交分析结果显示，有4个凋亡相关基因半胱天冬酶-6、脂肪酸合成酶配体基因、B淋巴细胞瘤-2（B—celllymphoma-2，BCL-2）和BCL-2样蛋白1（BCL-2like1，BCL-2L1）RNA水平发生明显改变。结论海水可以诱导细胞凋亡，可能与其诱导的凋亡相关基因表达改变有关。

英文摘要：

Objective To study the characteristics and molecular mechanisms of cell apopto- sis induced by seawater. Methods Endothelial cell of vessels （ECV） 304 cells were treated with seawater diluted by normal saline in different ratio with different time. Then the changes of morpho- logy and cell death of ECV304 cells were observed by fluorescence microscope after stained with 4＇,6-diamidino-2-phenylindole （DAH）. Then apoptosis and necrosis of the cells were analyzed by flow cytometry （FCM） after stained with Annexin V-fluorescein isothiocyanate （HTC） and propidium iodide （PI）. The expression levels of apoptosis related genes in ECV304 cells were detected by qPCR-array. Results ECV304 cells were shrunk at the early period of seawater treatment. Then the numbers of apoptosis and necrosis cells were increased. Seawater diluted by equal volume normal sa- line induced both apoptosis and necrosis when identified by fluorescence microscope and FCM. The identical numbers of apoptosis and necrosis cells were showed by FCM. The RNA levels of 4 apopto- sis related genes （CASP6, FASLG, BCL-2L1 and BCL-2） were changed apparently detected by qPCR-array. Conclusion Seawater may induce cell apoptosis. The mectlanisms maybe associated with the changes of the expression levels of apoptosis related genes in cells.