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中文摘要：
本研究旨在探讨动物源性沙门菌的血清型分布、生物被膜形成能力及其耐药性。从不同动物病料中分离细菌,以PCR方法鉴定沙门菌,结合玻片凝集法和16SrRNA序列测定确定沙门菌的血清型和分布,结晶紫染色定量法检测分离株的生物被膜形成能力,药敏试验检测分离菌对常用药物的敏感性。结果共分离鉴定出58株沙门菌,包括鸡白痢沙门菌、鼠伤寒沙门菌、肠炎沙门菌、丙型副伤寒沙门菌、乙型副伤寒沙门菌、都柏林沙门菌和阿哥那沙门菌等7种血清型,其中鸡群以鸡白痢沙门菌感染为主,肠炎沙门菌次之;水禽以鼠伤寒沙门菌感染为主。生物被膜测定结果显示51.72%的沙门菌分离株可形成生物被膜,其中83.33%的鼠伤寒沙门菌可形成生物被膜。20种抗生素（氨基糖苷类、磺胺类、喹诺酮类、林可酰胺类、氯霉素类、青霉素类、四环素类和头孢菌素类）敏感性试验表明所有菌株对林可霉素耐药,51.72%对4种及其以上抗生素耐药,并出现了1株对所有受试抗生素均耐药的鼠伤寒沙门菌。结果表明鸡白痢沙门菌、鼠伤寒沙门菌和肠炎沙门菌是为目前在家禽中分离的优势血清型;同时具有生物被膜形成能力和多重耐药性的沙门菌将对家禽疾病控制和公共卫生带来更大的威胁。
英文摘要：
The objective of this study was to investigate the distribution of serotypes, biofilm- forming ability and antimicrobial resistance of salmonella strains isolated from animals. Salmo- nella strains were isolated from diseased animals, identified by PCR combination with slide agglu- tination test and sequence analysis of 16S rRNA gene. Biofilm-forming ability of the isolates was detected by crystal violet assay, and antimicrobial resistance was determined by antibiotic suscep- tibility test. Fifty-eight strains were identified as Salmonella and belonged to seven subtypes, including Pullorum, Typhimurium, Enteritidis, Paratyphi-C, Paratyphi-B, Dublin and Agona. The chickens were mainly infected by S. pullorum, secondly by S. enteritidis, whereas the waterfowls were mainly infected by S. typhimurium. The results of biofilm formation test showed that 51.72% of the salmonella isolates could form biofiim, in which 83.3% of S. typhi- murium could form biofilm. The susceptibility test of 20 antibiotics （including Aminoglycosides, Sulfonamides, Quinolones, Lincosamides, Amphenicols, Penicillins, Tetracyclines and Cephalosporins） revealed that all of the strains were resistant to lincomycin, and 51.72% of them were resistant to four and more than four antimicrobials, in which a strain of S. typhimurium dis- played a high level of resistance to all test antibiotics. The results indicate that the dominant se- rotypes of Salmonella isolated from poultry are S. pullorum, S. typhimurium and S. enteriti- dis. The Salmonella with both biofilm-forming ability and multiple drug resistance will bring more serious threat to the control of poultry diseases and public health.
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Identification of Genes Responsible for Biofilm Formation or Virulence in Salmonella enterica Serova
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