中文摘要：

目的：观察IL-2＋IL-15组合体外培养方案对于NK、NKT和T细胞亚群的比例、表型、杀伤肿瘤细胞活性与黏附活性的影响，并初步探讨其作用机制。方法：采集天津医科大学附属肿瘤医院生物治疗科2012年5月至2012年7月期间收治的5例乳腺癌患者的外周血单个核细胞（peripheralbloodmononuclearcell，PBMC），用IL-2＋IL-15联合培养方案进行培养，观察培养15d后细胞的扩增倍数和淋巴细胞亚群比例变化，流式细胞术检测细胞免疫表型、细胞表面受体的表达，LDH释放法和CDl07a释放法检测不同细胞亚群对于HLA匹配或不匹配的靶肿瘤细胞系的杀伤活性，活细胞工作站检测总扩增产物对于不同靶肿瘤细胞系的黏附作用。结果：与扩增前相比，IL-2＋IL-15培养方案扩增15d后，NK细胞[（36．74±17．10）％VS（16．34±3．12）％，P〈0．05]、NKT细胞[（24．88±12．34）％vs（4．06±2．05）％，P〈0．05]比例显著增加，CD4’T细胞和Treg细胞比例显著降低（P〈0．05），CD8’T细胞比例显著升高（P〈0．05）；NK细胞表面活化受体NKp30[（92．384-7．19）％协（32．14-4-17．64）％，P〈0．05]、NKp44[（74．264-16．28）％Y．S（1．94±1．60）％，P〈0．05]、NKG2D[（98．58±1．28）％郴（66．50±24．84）％，P〈0．05]的表达率均显著升高，CDl6表达率显著降低[（85．124-7．66）％VS（95．604-2．53）％，P〈0．05]；NKT细胞、T细胞表面活化受体DNAM一1和NKG2D明显升高（P〈0．05）。总扩增产物、NK细胞和NKT细胞对HLA不匹配的靶肿瘤细胞的杀伤率均显著高于HLA匹配靶细胞系的杀伤（P〈0．05）；在共孵育至84min时，与HLA匹配的靶肿瘤细胞系相比，总扩增产物细胞与HLA不匹配的靶细胞系黏附结合数目显著增多[（4．804-0．53）”s（2．254-0．35）个，P〈0．05]。结论：IL-2＋IL-15组合方案在扩增NK细胞的同时，也能够有效扩增NKT细?

英文摘要：

Objective：To observe the effect of culture with IL-2 and IL-15 in vitro on the proportion, the cell pheno type, the cytotoxic activity against tumor ceils and the adhesion activity of NK cells, NKT cells and T cells subsets, and to discuss the possible mechanism. Methods ： Five patients with breast cancer were obtained in the Department of Biother apy, Tianjin Medical University Cancer Institute and Hospital from May 2012 to July 2012, and PBMCs of those patients were isolated by and cultured with the NK cells proliferation scheme contained IL-2 ＋ IL-5. The cell expansion fold andthe proportion of different lymphocyte subsets were observed after cultured by IL-2 and IL-15 for 15 d. The changes of the cell immunophenotype and cell surface receptors were detected by flow cytometry. The anti-tumor cytotoxicity against HLA-match or HLA-mismatch cancer cells among three lymphocyte subsets were measured using LDH cytotoxicity assay and CD107a release assay. Adhesion between the total expansion products and HLA-match or HLA-mismatch tumor cells was detected by Live Cell Station. Results： After expansion by IL-2 ＋ IL-15 for 15 days, compared with preamplification, the proportions of NK cells （ [ 36.74 ±17.10 % vs [ 16.34 -＋ 3.12 ] %, P 〈 0.05 ） and NKT cells （ [ 24.88 ± 12.34 ] % vs [4.06 ＋ 2.05 ] %, P 〈 0.05 ~ were significantly increased ; the proportions of CD4 ^＋ T cells and Treg cells were signifi- candy decreased （ P 〈 0.05 ）, the proportions of CD8 ^＋ T cells were signifieantly increased （ P 〈 0.05 ） ; the expressions of surface activation receptors NKp30 （U92.38 ＋7. 191% vs （32.14 ＋17.64）%, P〈0.05], NKp44 [（74.26±16.281% vs [1.94±1.601%, P〈0.05] and NKG2D （[98.58 ±1.28）% vs （66.50＋24.84）%, P〈0.05] ex pressed on NK cells were increased signifieantly, while CD16 was obviously decreased （ [85.12 ±7.66] % vs [95.60 ＋ 2.53 ] % , P 〈 0.05 ] ; the activation receptors DNAM-1 and NKG2D expressed on both NKT cells and T cells were signifi- ca