中文摘要：

目的观察microRNA-1（miR-1）对肝癌细胞系Hep3B死亡结构域沉默子（BAG4）的表达及细胞凋亡的影响。方法Westernblot检测肝癌组织中BAG4蛋白表达情况；将过表达miR-1的质粒转染肝癌细胞Hep3B。分别用RT-qPCR及Westernblot检测BAG4mRNA和蛋白的表达，流式细胞仪检测细胞凋亡；生物信息学软件分析miR-1和BAG43’UTR作用关系。结果BAG4蛋白在肝癌组织中的表达明显高于癌旁组织；Hep3B转染miR-1过表达质粒后，相较于阴性对照（NC）组，BAG4mRNA和蛋白水平的表达均有所降低；早期凋亡率明显增高。生物信息学分析提示BAG4是miR-1潜在靶基因。结论miR-1可以诱导肝癌细胞Hep3B凋亡,其作用可能和抑制BAG4表达有关。

英文摘要：

To investigate the effects of miR-1 on BAG4 expression and cell apoptosis in human hepatoma carcinoma cell line Hep3B, we detected the expressions of BAGS protein in HCC tissues and paired non-cancerous liver （PNL） tissues with Western blot. After miR-1 overexpressing plasmid was transfected into Hep3B, the expression levels of BAG4 mRNA and protein were measured by RT-qPCR and Western blot respectively, and the apoptosis ratio of Hep3B was detected by flow cytometry. The relationship of miR-1 and BAG4 were analyzed with bioinformatics method. Results showed that protein level of BAG4 in HCC tissue was much higher than that in PNL tissue. The miR-1 overexpressing group demonstrated much lower of BAG4 mRNA and protein levels, but higher level of cell apoptosis, as compared with negative control （NC） group. Bioinformatics analysis revealed BAG4 may be a potential target gene of miR-1. Therefore, we concluded miR-1 can induce apoptosis of Hep3B by downregulating BAGS expression.